The Key Laboratory of Biomimetic Sensing and Advanced Robot Technology, Anhui Province, Institute of Intelligent Machines, Chinese Academy of Science, Hefei, 230031 Anhui, PR China.
Colloids Surf B Biointerfaces. 2010 Oct 1;80(1):94-102. doi: 10.1016/j.colsurfb.2010.05.041. Epub 2010 Jun 1.
We reported a very simple, reliable, clean, nontoxic and eco-friendly biological method for the synthesis of semiconductor monoclinic Se nanoparticles by the Bacillus subtilis. The as-synthesized Se nanoparticles were spherical shaped with diameters ranging from 50 to 400nm. These spherical monoclinic Se nanoparticles can be transformed into highly anisotropic, one-dimensional (1D) trigonal structure after one day at the room temperature and grown from aqueous solutions. Furthermore, the obtained two kinds of Se nanomaterial crystals with high surface-to-volume ratio, good adhesive ability and biocompatibility have been employed as enhancing and settled materials for H(2)O(2) biosensor. The results show that the H(2)O(2) biosensor has high sensitivity and affinity for H(2)O(2). The detection limit for H(2)O(2) was found to be 8x10(-8)M. Different crystals of Se nanomaterials have no significant difference in electrochemical application.
我们报道了一种非常简单、可靠、清洁、无毒且环保的生物方法，通过枯草芽孢杆菌合成半导体单斜硒纳米粒子。所合成的硒纳米粒子呈球形，直径在 50 到 400nm 之间。这些球形单斜硒纳米粒子在室温下放置一天后可以转化为高度各向异性的一维（1D）三角结构，并从水溶液中生长。此外，所得到的两种具有高表面积与体积比、良好粘附能力和生物相容性的硒纳米材料晶体被用作 H（2）O（2）生物传感器的增强和固定材料。结果表明，H（2）O（2）生物传感器对 H（2）O（2）具有高灵敏度和亲和力。H（2）O（2）的检测限被发现为 8x10（-8）M。不同晶体的硒纳米材料在电化学应用中没有显著差异。
