Eseyin O A, Ebong P, Eyong E U, Umoh E, Agboke A
Faculty of Pharmacy, University of Uyo, P.O. Box 4274, Uyo, Akwa Ibom State, Nigeria.
Pak J Pharm Sci. 2010 Jul;23(3):341-3.
The leaf of Telfairia occidentalis has been found to possess hypoglycemic or antihyperglycemic effect. The hypoglycemic principle of the leaf is yet to be isolated. The aim of this study is to evaluate the hypoglycemic activity of some fractions of ethanolic leaf extract of Telfairia occidentalis in rat as a step toward activity directed isolation of the hypoglycemic component. Ethanolic leaf extract was successively extracted with ethyl acetate, butanol and ethanol to obtain ethyl acetate, butanol and ethanol fractions (I-III). The residue was taken as fraction IV. 250 mg/kg of the various extracts were orally administered to normoglycaemic and alloxan-induced diabetic albino rats. Blood glucose concentration was evaluated at 0, 1, 2 and 4 hours after treatment with One Touch glucometer. None of the fractions reduced glucose concentration in the normoglycaemic rats, while only ethyl acetate fraction lowered glucose concentration significantly at 2 and 4 hours (49.7 and 39.0%) compared to control value of 74.9 and 69.7%, respectively, in the diabetic rats. The results showed that the hypoglycemic component of the ethanolic leaf extract of the plant is contained in the ethyl acetate fraction.
已发现西非葫芦叶具有降血糖或抗高血糖作用。该叶的降血糖成分尚未分离出来。本研究的目的是评估西非葫芦乙醇叶提取物的某些馏分对大鼠的降血糖活性，作为朝着活性导向分离降血糖成分迈出的一步。乙醇叶提取物依次用乙酸乙酯、丁醇和乙醇萃取，得到乙酸乙酯、丁醇和乙醇馏分（I - III）。残渣作为馏分IV。将250mg/kg的各种提取物口服给予血糖正常和四氧嘧啶诱导的糖尿病白化大鼠。用稳豪血糖仪在治疗后0、1、2和4小时评估血糖浓度。在血糖正常的大鼠中，没有一个馏分降低血糖浓度，而在糖尿病大鼠中，只有乙酸乙酯馏分在2小时和4小时时显著降低血糖浓度（分别为49.7%和39.0%），而对照值分别为74.9%和69.7%。结果表明，该植物乙醇叶提取物的降血糖成分存在于乙酸乙酯馏分中。
