Department of Molecular Medicine, The Institute of Experimental Medicine, Istanbul University, 34280, Capa Istanbul, Turkey.
Mol Biol Rep. 2011 Feb;38(2):887-91. doi: 10.1007/s11033-010-0181-4. Epub 2010 Jun 22.
Myeloperoxidase is a lysosomal enzyme of polymorphonuclear leucocytes that contributes to inflammatory responses. In previous studies it was shown that MPO was synthesized in atherosclerotic lesions responsible of lipoprotein oxidations. We aimed to determine the MPO -463 G/A gene polymorphism distribution in Turkish population and evaluate the effects of it on myeloperoxidase levels. There were 100 myocardial infarct patients and 100 healthy control subjects in our study. MPO polymorphism was studied by using PCR-RFLP technique and MPO levels were measured by ELISA. It was shown that MPO levels were increasing in patients after myocardial infarct event but there were no effect of MPO -463 G/A polymorphism on MPO levels. It was also found that serum total cholesterol and LDL-cholesterol levels and smoking was contributing factors in increments of MPO enzymes. We observed that MPO levels were increased in CAD but there were no effect of MPO -463 G/A polymorphism on MPO levels.
髓过氧化物酶是一种存在于多形核白细胞溶酶体中的酶，参与炎症反应。先前的研究表明，MPO 是负责脂蛋白氧化的动脉粥样硬化病变中合成的。我们旨在确定土耳其人群中 MPO-463G/A 基因多态性的分布，并评估其对髓过氧化物酶水平的影响。在我们的研究中，有 100 例心肌梗死患者和 100 例健康对照者。采用 PCR-RFLP 技术研究 MPO 多态性，用 ELISA 法测定 MPO 水平。结果表明，心肌梗死后患者 MPO 水平升高，但 MPO-463G/A 多态性对 MPO 水平无影响。还发现血清总胆固醇和 LDL 胆固醇水平以及吸烟是 MPO 酶升高的因素。我们观察到 CAD 患者的 MPO 水平升高，但 MPO-463G/A 多态性对 MPO 水平无影响。
