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连续亲和色谱法,采用磁稳定流化床。

Continuous affinity chromatography using a magnetically stabilized fluidized bed.

机构信息

Department of Chemical Engineering University of Pennsylvania Philadelphia, Pennsylvania.

出版信息

Biotechnol Prog. 1985 Jun;1(2):95-103. doi: 10.1002/btpr.5420010206.

DOI:10.1002/btpr.5420010206
PMID:20568146
Abstract

A new processing tool, the magnetically stabilized fluidized bed, offers opportunities for efficient fluid-solids contact.The bubbling and mixing normally seen in a fluidized bed are eliminated, and continuous countercurrent contact is possible. This technique is especially suitable for biochemical separations, where cellular debris would clog an ordinary fixed bed. Preliminary modeling and experimental work on such a system is discussed, along with details of a relatively simple experimental system.

摘要

一种新型的加工工具,即磁稳定流化床,为高效的固液接触提供了机会。流化床中通常出现的鼓泡和混合现象被消除了,并且可以实现连续的逆流接触。这种技术特别适用于生化分离,因为普通的固定床会被细胞碎片堵塞。本文讨论了这种系统的初步建模和实验工作,并详细介绍了一个相对简单的实验系统。

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1
Continuous affinity chromatography using a magnetically stabilized fluidized bed.连续亲和色谱法,采用磁稳定流化床。
Biotechnol Prog. 1985 Jun;1(2):95-103. doi: 10.1002/btpr.5420010206.
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Adv Biochem Eng Biotechnol. 2018;165:115-178. doi: 10.1007/10_2017_12.
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Magnetic techniques for the isolation and purification of proteins and peptides.用于蛋白质和肽分离与纯化的磁性技术。
Biomagn Res Technol. 2004 Nov 26;2(1):7. doi: 10.1186/1477-044X-2-7.
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Performance of a magnetically stabilized bed reactor with immobilized yeast cells.
固定化酵母细胞的磁稳定床反应器性能
Appl Biochem Biotechnol. 1996 May;59(2):187-98. doi: 10.1007/BF02787820.