Zheng Juan, Liu Kan, Kang Zhimin, Cai Jianmei, Liu Wenwu, Xu Weigang, Li Runping, Tao Hengyi, Zhang John H, Sun Xuejun
Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China.
Undersea Hyperb Med. 2010 May-Jun;37(3):185-92.
Exposure to high oxygen concentrations leads to acute lung injury, including lung tissue and alveolar edema formation, congestion, intra-alveolar hemorrhage, as well as endothelial and epithelial cell apoptosis or necrosis. Several studies have reported that molecular hydrogen is an efficient antioxidant by gaseous rapid diffusion into tissues and cells. Moreover, consumption of water with dissolved molecular hydrogen to a saturated level (hydrogen water) prevents stress-induced cognitive decline in mice and superoxide formation in mice. The purpose of the present study was to investigate the effect of saturated hydrogen saline on pulmonary injury-induced exposure to >98% oxygen at 2.5 ATA for five hours. Adult male Sprague-Dawley (SD) rats were randomly divided into three groups: control group, saline group and saturated hydrogen saline group. Hematoxylin and eosin (H&E) staining were used to examine histological changes. The lung wet to dry (W/D) weight ratio was calculated. The concentration of protein and total cell counts in bronchoalveolar lavage fluid (BALF) were measured. Lactate dehydrogenase (LDH) in serum and BALF were measured by spectrophotometer. The light microscope findings showed that saturated hydrogen saline reduced the impairment when compared with the saline group: Saturated hydrogen saline decreased lung edema, reduced LDH activity in BALF and serum, and decreased total cells and protein concentration in BALF. These results demonstrated that saturated hydrogen saline alleviated hyperoxia-induced pulmonary injury, which was partly responsible for the inhibition of oxidative damage.
暴露于高氧浓度会导致急性肺损伤，包括肺组织和肺泡水肿形成、充血、肺泡内出血，以及内皮细胞和上皮细胞凋亡或坏死。多项研究报告称，分子氢通过快速扩散进入组织和细胞而成为一种有效的抗氧化剂。此外，饮用溶解有分子氢至饱和水平的水（富氢水）可预防小鼠应激诱导的认知能力下降以及小鼠体内超氧化物的形成。本研究的目的是探讨饱和氢盐水对在2.5个绝对大气压下暴露于>98%氧气5小时所引起的肺损伤的影响。成年雄性Sprague-Dawley（SD）大鼠被随机分为三组：对照组、盐水组和饱和氢盐水组。采用苏木精-伊红（H&E）染色检查组织学变化。计算肺湿重与干重（W/D）比值。测量支气管肺泡灌洗液（BALF）中的蛋白质浓度和总细胞计数。用分光光度计测量血清和BALF中的乳酸脱氢酶（LDH）。光学显微镜检查结果显示，与盐水组相比，饱和氢盐水减轻了损伤：饱和氢盐水减轻了肺水肿，降低了BALF和血清中的LDH活性，并降低了BALF中的总细胞数和蛋白质浓度。这些结果表明，饱和氢盐水减轻了高氧诱导的肺损伤，这部分归因于对氧化损伤的抑制作用。
