[结核感染T细胞检测在肺及肺外结核病诊断中的应用]

[Quantiferon-TB Gold In-Tube test in the diagnosis of pulmonary and extra-pulmonary tuberculosis].

作者信息

Garcia-Gasalla Mercedes, Fernández-Baca Victoria, Mir-Viladrich Isabel, Cifuentes-Luna Carmen, Campins-Roselló Antoni, Payeras-Cifre Antoni, Serrano-Bujalance Araceli, Ortiz-Monjo Alicia, Pons-Vives Salvador, Gallegos-Alvarez Carmen

机构信息

Servicio de Medicina Interna, Hospital Son Llàtzer, Mallorca, España.

出版信息

Enferm Infecc Microbiol Clin. 2010 Dec;28(10):685-9. doi: 10.1016/j.eimc.2010.01.008. Epub 2010 Jun 8.

DOI:10.1016/j.eimc.2010.01.008

PMID:20570416

Abstract

INTRODUCTION

The Interferon-γ in vitro detection tests could be a useful tool in the diagnosis of active tuberculosis compared to Mycobacterium tuberculosis (MTB).

METHODS

The QuantiFERON-TB-Gold in Tube (QFG-IT) test was performed on the blood of 118 patients with active tuberculosis and the results compared with the tuberculin test.

RESULTS

The QFG-IT test was positive in 94 cases (79.7%), negative in 17 (14.4%) and indeterminate in 7 (5.9%). A negative or indeterminate QFG-IT test was more common in older patients (P=0.017) and in cases with negative smear tests (P=0.041). The kappa agreement between the tuberculin and QFG-IT tests was 74.5% with a kappa value of 0.45 (SE:0.136). Thirteen of the patients studied were infected with HIV and the tuberculin was positive in 5 of the 12 cases (38.5%) in whom it was performed, with the QFG-IT being positive in 9/13 (69.2%).

CONCLUSIONS

The QFG-IT test may be a useful complimentary tool to the tuberculin test in the diagnosis of tuberculosis.

摘要

引言

与结核分枝杆菌（MTB）相比，干扰素-γ体外检测试验可能是诊断活动性结核病的一种有用工具。

方法

对118例活动性结核病患者的血液进行管内QuantiFERON-TB-金标（QFG-IT）试验，并将结果与结核菌素试验进行比较。

结果

QFG-IT试验94例（79.7%）为阳性，17例（14.4%）为阴性，7例（5.9%）结果不确定。QFG-IT试验阴性或结果不确定在老年患者（P = 0.017）和涂片试验阴性的病例中更为常见（P = 0.041）。结核菌素试验与QFG-IT试验的kappa一致性为74.5%，kappa值为0.45（标准误：0.136）。所研究的患者中有13例感染了HIV，在进行结核菌素试验的12例患者中，5例（38.5%）呈阳性，而QFG-IT试验在13例中的9例（69.2%）呈阳性。