The Materials Science and Engineering Program, The Iby and Aladar Fleischman Faculty of Engineering, Tel-Aviv University, Ramat Aviv, Tel-Aviv 69978, Israel.
Acta Biomater. 2010 Nov;6(11):4430-8. doi: 10.1016/j.actbio.2010.06.003. Epub 2010 Jun 8.
Millions of people are stricken with the degenerative joint disease known as osteoarthritis. Osteoarthritis is associated with biochemical and mechanical processes, and is characterized by loss of articular cartilage and hypertrophy of bone. As cartilage and bone particles are released into the synovial fluid, a variety of biomarkers have been suggested for the analysis of this fluid. Here we have developed a method for isolating bone and cartilage wear particles suspended in the synovial fluid of the hip, knee and ankle joints of humans, based on specific magnetization of collagens I and II. Bio-ferrography is used to capture the particles on glass slides, allowing microscopic, chemical and statistical analyses. The relations between the level of the disease and the number, dimensions, shape and chemical composition of the particles were established. The method, which was found to be sensitive and reliable, can easily be extended to other applications, such as diagnosis of cancer and infectious diseases, determination of the efficacy of drugs or optimization of implants.
数以百万计的人患有退行性关节疾病，即骨关节炎。骨关节炎与生化和机械过程有关，其特征是关节软骨丧失和骨肥大。随着软骨和骨颗粒释放到滑液中，已经提出了多种生物标志物来分析这种液体。在这里，我们基于 I 型和 II 型胶原的特异性磁化，开发了一种从人髋关节、膝关节和踝关节的滑液中分离悬浮的骨和软骨磨损颗粒的方法。生物铁谱法用于将颗粒捕获在载玻片上，从而可以进行微观、化学和统计分析。确定了疾病程度与颗粒数量、尺寸、形状和化学成分之间的关系。该方法灵敏可靠，易于扩展到其他应用，如癌症和传染病的诊断、药物疗效的确定或植入物的优化。
