微绒毛的粘弹性特性取决于细胞表面分子。
The viscoelastic properties of microvilli are dependent upon the cell-surface molecule.
作者信息
Python Johanne L, Wilson Kristal O, Snook Jeremy H, Guo Bin, Guilford William H
机构信息
Department of Biomedical Engineering, University of Virginia, Box 800759, Charlottesville, VA 22908, USA.
出版信息
Biochem Biophys Res Commun. 2010 Jul 2;397(3):621-5. doi: 10.1016/j.bbrc.2010.06.012. Epub 2010 Jun 4.
We studied at nanometer resolution the viscoelastic properties of microvilli and tethers pulled from myelogenous cells via P-selectin glycoprotein ligand 1 (PSGL-1) and found that in contrast to pure membrane tethers, the viscoelastic properties of microvillus deformations are dependent upon the cell-surface molecule through which load is applied. A laser trap and polymer bead coated with anti-PSGL-1 (KPL-1) were used to apply step loads to microvilli. The lengthening of the microvillus in response to the induced step loads was fitted with a viscoelastic model. The quasi-steady state force on the microvillus at any given length was approximately fourfold lower in cells treated with cytochalasin D or when pulled with concanavalin A-coated rather than KPL-1-coated beads. These data suggest that associations between PSGL-1 and the underlying actin cytoskeleton significantly affect the early stages of leukocyte deformation under flow.
我们以纳米分辨率研究了通过P-选择素糖蛋白配体1（PSGL-1）从骨髓细胞中拉出的微绒毛和系链的粘弹性特性，发现与纯膜系链不同，微绒毛变形的粘弹性特性取决于施加负荷所通过的细胞表面分子。使用激光阱和包被有抗PSGL-1（KPL-1）的聚合物珠对微绒毛施加阶跃负荷。用粘弹性模型拟合微绒毛对诱导的阶跃负荷的伸长情况。在用细胞松弛素D处理的细胞中，或者当用伴刀豆球蛋白A包被而非KPL-1包被的珠子拉动时，在任何给定长度下微绒毛上的准稳态力大约低四倍。这些数据表明，PSGL-1与潜在的肌动蛋白细胞骨架之间的关联显著影响流动状态下白细胞变形的早期阶段。
Zotero 插件