Endoscopy Department, João de Barros Barreto University Hospital, 66073-000, Belém, Pará, Brazil.
World J Gastroenterol. 2010 Jun 28;16(24):3033-9. doi: 10.3748/wjg.v16.i24.3033.
To establish whether virulence factor genes vacA and cagA are present in Helicobacter pylori (H. pylori) retrieved from gastric mucosa and dental plaque in patients with dyspepsia.
Cumulative dental plaque specimens and gastric biopsies were submitted to histological examination, rapid urease test and polymerase chain reaction (PCR) assays to detect the presence of cagA and vacA polymorphisms.
Detection of H. pylori from dental plaque and gastric biopsy samples was greater by PCR compared to histological examination and the rapid urease test. DNA from H. pylori was detected in 96% of gastric mucosa samples and in 72% of dental plaque samples. Sixty-three (89%) of 71 dental plaque samples that were H. pylori-positive also exhibited identical vacA and cagA genotypes in gastric mucosa. The most common genotype was vacAs1bm1 and cagA positive, either in dental plaque or gastric mucosa. These virulent H. pylori isolates were involved in the severity of clinical outcome.
These pathogenic strains were found simultaneously in dental plaque and gastric mucosa, which suggests that gastric infection is correlated with the presence of H. pylori in the mouth.
确定从消化不良患者胃黏膜和牙菌斑中分离出的幽门螺杆菌（H. pylori）是否存在毒力因子基因 vacA 和 cagA。
收集累积的牙菌斑标本和胃活检组织，进行组织学检查、快速尿素酶试验和聚合酶链反应（PCR）检测，以检测 cagA 和 vacA 多态性的存在。
与组织学检查和快速尿素酶试验相比，PCR 检测到牙菌斑和胃活检样本中 H. pylori 的存在更多。H. pylori 的 DNA 分别在 96%的胃黏膜样本和 72%的牙菌斑样本中被检测到。71 个牙菌斑样本中有 63 个（89%）为 H. pylori 阳性，在胃黏膜中也表现出相同的 vacA 和 cagA 基因型。最常见的基因型是 vacAs1bm1 和 cagA 阳性，无论是在牙菌斑还是胃黏膜中。这些毒力 H. pylori 分离株与临床结果的严重程度有关。
这些致病菌株同时存在于牙菌斑和胃黏膜中，这表明胃感染与口腔中 H. pylori 的存在有关。
