Max Planck Institute for Terrestrial Microbiology, Department of Biogeochemistry, Marburg, Germany.
ISME J. 2010 Dec;4(12):1603-7. doi: 10.1038/ismej.2010.82. Epub 2010 Jun 24.
Little is known about population dynamics and contribution of specific taxa to methane oxidation in flooded rice paddies. In this article we investigate the succession of methanotrophs in oxygen-methane counter-gradients. We used a gradient microcosm system that simulates oxic-anoxic interfaces of a water-saturated paddy soils, and measured pmoA-based (gene encoding particulate methane monooxygenase) terminal restriction fragment length polymorphism (T-RFLP) profiles at both the transcription (mRNA) and the population (DNA) levels. The DNA T-RFLP profiles indicated that the methanotrophic community present clearly differed from the active methanotrophic community. We observed a succession of the methanotrophic community over time without any direct effect of pore water chemistry on the community structure. Both the total population and the active subpopulation changed with time, whereas methane oxidation rates remained nearly constant. Hence, we suggest that a diverse microbial seed bank of methanotrophs is important in maintaining the function in a dynamic ecosystem.
关于在淹水稻田中甲烷氧化的种群动态和特定分类群的贡献，人们知之甚少。在本文中，我们研究了在氧-甲烷反梯度中甲烷氧化菌的演替。我们使用了一个梯度微宇宙系统来模拟饱和稻田的好氧-缺氧界面，并在转录（mRNA）和种群（DNA）水平上测量基于 pmoA 的末端限制性片段长度多态性（T-RFLP）图谱。DNA T-RFLP 图谱表明，存在的甲烷氧化菌群与活跃的甲烷氧化菌群明显不同。我们观察到甲烷氧化菌群随时间的演替，而没有任何孔隙水化学对群落结构的直接影响。总种群和活跃亚种群都随时间而变化，而甲烷氧化速率几乎保持不变。因此，我们认为，甲烷氧化菌的多样化微生物种子库对于维持动态生态系统的功能很重要。
