Xu Wen-bin, Guo Qiao-sheng, Wang Chang-lin
Institute of Chinese Medicinal Materials, Nanjing Agricultural University, China.
Zhongguo Zhong Yao Za Zhi. 2006 Jan;31(1):18-21.
To investigate the genetic diversity of Chrysanthemum morifolium at the level of molecular biology.
The total genomic DNA was extracted from medicinal chrysanthemums by 2% CTAB method. And the genetic diversity of 22 C. morifolium accessions was tested by RAPD marks. The NTSYS software was used to analyze the marks.
26 10-mer arbitrary primers were found to acquire polymophic results. A total of 233 bands were amplified, of which 89.7% bands were found to be polymophic. 8.04 polymophic bands were amplified by each primer on the average. The results of cluster analysis by using UPGMA method showed that all the tested accessions could be differentiated by RAPD marks.
There actually existed much genetic diversity at the molecular level among the germplasm resources of C. morifolium. RAPD marks could be effective tools to construct DNA fingerprintings of C. morifolium. The differences between the tested chrysanthemums are related to the environments. However, it was affected by genetic facters more significantly.
在分子生物学水平上研究菊花的遗传多样性。
采用2% CTAB法从药用菊花中提取总基因组DNA。利用RAPD标记检测22份菊花种质的遗传多样性。使用NTSYS软件对标记进行分析。
发现26条10聚体随机引物能获得多态性结果。共扩增出233条带，其中89.7%的带具有多态性。平均每条引物扩增出8.04条多态性带。采用UPGMA法进行聚类分析的结果表明，所有供试种质均可通过RAPD标记区分。
菊花种质资源在分子水平上确实存在丰富的遗传多样性。RAPD标记可作为构建菊花DNA指纹图谱的有效工具。供试菊花之间的差异与环境有关。然而，其受遗传因素的影响更为显著。
