Fu Yong, Lei Peng, Han Yu-mei, Yan Dan
Chengdu University of TCM, Chengdu 610075, China.
Zhong Yao Cai. 2010 Feb;33(2):267-72.
To study the technological parameters of the purification process of saponins with macroporous adsorption resin.
The adsorptive characteristics and elutive parameters of the process were studied by taking the elutive and purified ratio of saponins as markers. Bacteriostasis activity of each parts eluted was evaluated by the mean of cup-plate method.
13.6 mL of the extraction of sapindus saponin (crude drugs 0.01 g/mL) was purified with a column of macroporous adsorption resin (phi15 mm x H90 mm, dry weight 2.5 g) and washed with 3BV of distilled water, then eluted with 3BV of 30% ethanol and 3BV of 70% ethanol, most of saponins were collected in the 70% ethanol.
With macroporous adsorption resin adsorbing and purifying, the elutive ratio of saponins was 93.8% and the purity reached 250.1%. So this process of applying macroporous adsorption resin to adsorb and purify saponins is feasible, and supplies reference to the purification of other types of saponin.
研究大孔吸附树脂纯化皂苷工艺的技术参数。
以皂苷的洗脱率和纯化率为指标，研究该工艺的吸附特性和洗脱参数。采用杯碟法测定各洗脱部位的抑菌活性。
用1根大孔吸附树脂柱（φ15mm×H90mm，干重2.5g）对13.6mL无患子皂苷提取物（生药0.01g/mL）进行纯化，先用3倍柱体积蒸馏水冲洗，再用3倍柱体积30%乙醇和3倍柱体积70%乙醇洗脱，大部分皂苷收集于70%乙醇洗脱部位。
大孔吸附树脂吸附纯化皂苷，皂苷洗脱率为93.8%，纯度达250.1%。该大孔吸附树脂吸附纯化皂苷工艺可行，为其他类型皂苷的纯化提供了参考。
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