[Studies on separation and purification of total saponin from flower buds of Panax notoginseng by macroporous resins].

OBJECTIVE

To optimize the technical parameters of separation and purification of total saponin from flower buds of Panax notoginseng by macroporous resins.

METHODS

Based on absorptive capacity and elution efficiency of total saponin from flower buds of Panax notoginseng, the best type of resin and the conditions to separate and purify total saponin by macroporous resins were optimized.

RESULTS

The AB-8 resin was chosen, washed with 3 BV of 70% ethanol, and with the absorption and elution velocity of flow 2BV/h. The optimal concentration of the raw material was 0. 2 g/ml. The purity of total saponin obtained was 87.60%.

CONCLUSION

Using AB-8 resin to separate and purify total saponin from flowers of Panax notoginseng is feasible.