Wei Li, Zhou Hao
Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Zhong Yao Cai. 2008 Sep;31(9):1418-21.
To optimize the technical parameters of separation and purification of total saponin from flower buds of Panax notoginseng by macroporous resins.
Based on absorptive capacity and elution efficiency of total saponin from flower buds of Panax notoginseng, the best type of resin and the conditions to separate and purify total saponin by macroporous resins were optimized.
The AB-8 resin was chosen, washed with 3 BV of 70% ethanol, and with the absorption and elution velocity of flow 2BV/h. The optimal concentration of the raw material was 0. 2 g/ml. The purity of total saponin obtained was 87.60%.
Using AB-8 resin to separate and purify total saponin from flowers of Panax notoginseng is feasible.
优化大孔树脂分离纯化三七花蕾总皂苷的工艺参数。
基于三七花蕾总皂苷的吸附量和洗脱效率，优化大孔树脂分离纯化总皂苷的最佳树脂类型及条件。
选择AB-8树脂，用3倍柱体积的70%乙醇洗脱，吸附和洗脱流速为2倍柱体积/小时。原料最佳浓度为0.2克/毫升。所得总皂苷纯度为87.60%。
采用AB-8树脂分离纯化三七花总皂苷是可行的。
