形成 Ca/Cd 联合毒性对秀丽隐杆线虫寿命的影响。
Formation of a combined Ca/Cd toxicity on lifespan of nematode Caenorhabditis elegans.
机构信息
Key Laboratory of Developmental Genes and Human Disease in Ministry of Education, Institute of Life Sciences, Southeast University, Nanjing 210009, China.
出版信息
Ecotoxicol Environ Saf. 2010 Sep;73(6):1221-30. doi: 10.1016/j.ecoenv.2010.05.002. Epub 2010 Jun 30.
We investigated the possible formation of combined toxicity from Ca/Cd exposure on nematode lifespan. Ca exposure at concentrations more than 1.56 mM significantly reduced lifespan, accelerated aging-related declines, and induced severe stress response in wild-type nematodes. Combined Ca (25 mM)/Cd (200 microM) exposure decreased the lifespans compared to Cd (200 microM) exposure; whereas no lifespan differences were found between Ca (1.56 mM)/Cd (200 microM) exposure and Cd (200 microM) exposure. Combined Ca (25 mM)/Cd (200 microM) exposure caused a more significant induction of hsp-16.2::gfp expression, and a more severe increase in oxidative damage than Cd (200 microM) exposure. Moreover, mutation of mev-1, encoding a subunit of succinate dehydrogenase cytochrome b, enhanced the combined Ca/Cd toxicity on lifespan. Furthermore, mutation of daf-16, encoding a fork-head-family transcription factor, enhanced the combined Ca/Cd toxicity on lifespan, and mutation of daf-2, encoding an insulin receptor-like protein, alleviated the combined Ca/Cd toxicity on lifespan.
我们研究了 Ca/Cd 暴露对线虫寿命可能产生的联合毒性。暴露于浓度超过 1.56mM 的 Ca 显著缩短了线虫的寿命，加速了与衰老相关的下降，并诱导了野生型线虫的严重应激反应。与 Cd（200μM）暴露相比，Ca（25mM）/Cd（200μM）联合暴露降低了寿命；而 Ca（1.56mM）/Cd（200μM）暴露与 Cd（200μM）暴露之间没有寿命差异。Ca（25mM）/Cd（200μM）联合暴露引起 hsp-16.2::gfp 表达的诱导更为显著，氧化损伤的增加也比 Cd（200μM）暴露更为严重。此外，编码琥珀酸脱氢酶细胞色素 b 亚基的 mev-1 突变增强了 Ca/Cd 联合毒性对寿命的影响。此外，编码叉头家族转录因子的 daf-16 突变增强了 Ca/Cd 联合毒性对寿命的影响，而编码胰岛素受体样蛋白的 daf-2 突变减轻了 Ca/Cd 联合毒性对寿命的影响。
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