Key Laboratory of Developmental Genes and Human Disease in Ministry of Education, Department of Biochemistry and Molecular Biology, Southeast University Medical School, Nanjing 210009, China.
Ecotoxicol Environ Saf. 2010 Mar;73(3):423-9. doi: 10.1016/j.ecoenv.2009.12.014. Epub 2009 Dec 31.
In nematodes, 10 J/m(2)/min of UV irradiation induced a mild reproductive toxicity. Pre-treatment with UV irradiation at 10 J/m(2)/min suppressed the formation of reproductive defects, and activated a noticeable reduction of percentage of population with hsp-16.2::gfp expression, an obvious elevation of superoxide dismutase activities, and decrease of oxidative damage in 50 and 100 microM Cd exposed nematodes; however, pre-treatment with UV irradiation at 20 J/m(2)/min caused a significant decrease of brood sizes or increase of generation times in Cd-exposed nematodes. Pre-treatment with mild UV irradiation did not suppress the formation of reproductive defects in 150 microM Cd-exposed nematodes. Furthermore, the adaptive response to reproductive toxicity from Cd exposure was not observed in a reactive oxygen species sensitive mev-1(kn1) mutant. Therefore, pre-treatment with mild UV irradiation triggers the resistance to reproductive toxicity from Cd exposure by at least partially inducing adaptation to oxidative stress and through a mev-1-dependent pathway.
在线虫中，10 J/m(2)/min 的紫外线辐照会引起轻微的生殖毒性。10 J/m(2)/min 的紫外线辐照预处理可抑制生殖缺陷的形成，并显著降低 hsp-16.2::gfp 表达的群体比例，超氧化物歧化酶活性明显升高，50 和 100 microM Cd 暴露的线虫中的氧化损伤减少；然而，20 J/m(2)/min 的紫外线辐照预处理会导致 Cd 暴露的线虫的产卵量显著减少或世代时间延长。150 microM Cd 暴露的线虫中，轻度紫外线辐照预处理不能抑制生殖缺陷的形成。此外，在活性氧敏感的 mev-1(kn1)突变体中，没有观察到对 Cd 暴露生殖毒性的适应性反应。因此，轻度紫外线辐照预处理通过至少部分诱导对氧化应激的适应和通过依赖于 mev-1 的途径触发对 Cd 暴露生殖毒性的抗性。
