Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental Botany, Sokolovská 6, CZ-77200 Olomouc, Czech Republic.
Cytometry A. 2010 Jul;77(7):635-42. doi: 10.1002/cyto.a.20915.
Correct information on genome size is important in many areas of research. For a long time, scientists have been struggling to understand the reason for the huge variation in eukaryotic genome size and its biological significance. More recently, the knowledge on genome size has become important to structure genome sequencing projects as their scale and cost depend on genome size. Despite the fact that the first estimates of genome size in eukaryotes were made more than 50 years ago, we are still not quite sure about the exact genome size in practically all animal and plant species. Moreover, different estimates continue to be published for the same species. These discrepancies compromise data comparison and interpretation and point to methodological problems, which include standardization. This article assesses the current state of DNA reference standards for flow cytometry and the issues related to their calibration.
正确的基因组大小信息在许多研究领域都很重要。长期以来，科学家们一直在努力理解真核生物基因组大小巨大变化的原因及其生物学意义。最近，基因组大小的知识对于结构基因组测序项目变得重要，因为它们的规模和成本取决于基因组大小。尽管早在 50 多年前就对真核生物的基因组大小进行了首次估计，但我们仍然不能确定几乎所有动植物物种的确切基因组大小。此外，对于同一物种，还在不断发表不同的估计值。这些差异影响了数据的比较和解释，并指出了包括标准化在内的方法学问题。本文评估了目前用于流式细胞术的 DNA 参考标准的状况，以及与它们的校准相关的问题。
