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流式细胞术参考标准及其在核DNA含量比较研究中的应用。

Reference standards for flow cytometry and application in comparative studies of nuclear DNA content.

作者信息

Tiersch T R, Chandler R W, Wachtel S S, Elias S

机构信息

Department of Biology, Memphis State University, Tennessee 38152.

出版信息

Cytometry. 1989 Nov;10(6):706-10. doi: 10.1002/cyto.990100606.

DOI:10.1002/cyto.990100606
PMID:2582960
Abstract

Nuclear DNA mass in cells from a reference species can be used to obtain high-resolution estimates of DNA mass from a target species. In our study of DNA mass in cells from 45 selected species, representing each of the major vertebrate classes, we have obtained values of from 1.5 to 110.0 pg of DNA. Because values in or near this range would be expected in the study of nuclear DNA mass in vertebrates and other organisms, the species in this report can provide a useful catalogue of references for comparative studies of DNA.

摘要

来自参考物种细胞中的核DNA质量可用于获得目标物种DNA质量的高分辨率估计值。在我们对代表每个主要脊椎动物类别的45个选定物种细胞中的DNA质量进行的研究中,我们获得了1.5至110.0皮克DNA的值。由于在脊椎动物和其他生物体的核DNA质量研究中预计会出现该范围或接近该范围的值,本报告中的物种可为DNA的比较研究提供有用的参考目录。

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