Department of Prosthodontics, Faculty of Dentistry, The Hebrew University of Jerusalem, Israel.
J Biomed Mater Res B Appl Biomater. 2010 Aug;94(2):367-371. doi: 10.1002/jbm.b.31662.
Frequently provisional restorations require long-term permanence in the oral cavity, thus an antibacterial effect is desirable. We hypothesized that this effect may be achieved by incorporating polyethyleneimine (PEI) nanoparticles into provisional cements.
The nanoparticles antibacterial effect incorporated at 0.5%, 1%, and 2% w/w into provisional cement, was studied in vitro. The antibacterial effect against Streptococcus mutans and Enterococcus faecalis was tested using direct contact test. The data was analyzed using the ANOVA test, with the Dunnett test for multiple pairwise comparisons.
A strong antibacterial effect was evident in all test groups after an aging period of 14 days against S. mutans and E. faecalis (p < 0.05). A significant effect was found between study groups 0.5% w/w and 1% w/w group, as well as between study groups 0.5% w/w and 2% w/w for E. faecalis (p < 0.05). No significant difference was found between study groups 1% w/w and 2% w/w. The growth rate graphs depict an effective bacteria inhibition starting from 1% w/w.
PEI nanoparticles incorporated at low concentrations in a provisional cement exhibit antibacterial effect against S. mutans and E. faecalis for a period of 14 days. The minimum effective concentration suggested is 1% w/w.
Incorporation of nanoparticles may prevent caries and inflammation, and thereby improve the results of the prosthetic treatment. Further investigation is necessary on the effect on mechanical properties and clinical relevance.
临时修复体经常需要在口腔中长期使用，因此需要具有抗菌效果。我们假设通过将聚乙烯亚胺（PEI）纳米粒子加入到临时粘接剂中可以达到这种效果。
研究了将纳米粒子以 0.5%、1%和 2%w/w 的比例加入临时粘接剂中对 Streptococcus mutans 和 Enterococcus faecalis 的抗菌效果。使用直接接触试验测试抗菌效果。使用方差分析（ANOVA）检验进行数据分析，并进行多重两两比较的 Dunnett 检验。
在 14 天老化期后，所有测试组对 S. mutans 和 E. faecalis 均表现出强烈的抗菌效果（p<0.05）。在 0.5%w/w 和 1%w/w 组以及 0.5%w/w 和 2%w/w 组之间，E. faecalis 存在显著差异（p<0.05）。1%w/w 和 2%w/w 组之间无显著差异。生长率图表显示，从 1%w/w 开始，细菌抑制效果有效。
PEI 纳米粒子以低浓度加入临时粘接剂中对 S. mutans 和 E. faecalis 具有抗菌效果，建议的最低有效浓度为 1%w/w。
纳米粒子的加入可以预防龋齿和炎症，从而改善修复治疗的效果。需要进一步研究其对机械性能和临床相关性的影响。
