诺维科夫肝癌细胞核RNA中N6,O2-二甲基腺苷的特性分析
Characterization of N6, O2-dimethyladenosine from nuclear RNA of Novikoff hepatoma.
作者信息
Crain P F, Choi Y C, Busch H, McCloskey J A
出版信息
Nucleic Acids Res. 1978 Mar;5(3):771-6. doi: 10.1093/nar/5.3.771.
Abstract
A modified nucleoside was isolated from low molecular weight nuclear RNA of Novikoff hepatoma, the nucleotide sequence of which was reported earlier. The structure of this novel nucleoside was shown to be N6, O2'-dimethyladenosine (m6Am) by mass spectrometry of its trimethylsilyl derivative and by comparison with the mass spectra of N6- and O-2' monmethyl model compounds. The complete characterization was carried out using 0.04 A260 units (2 microgram) of m6Am.
摘要
从诺维科夫肝癌的低分子量核RNA中分离出一种修饰核苷，其核苷酸序列已于此前报道。通过对其三甲基硅烷基衍生物进行质谱分析，并与N6-和O-2'单甲基模型化合物的质谱进行比较，表明这种新型核苷的结构为N6，O2'-二甲基腺苷（m6Am）。使用0.04 A260单位（2微克）的m6Am进行了完整的表征。
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