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诺维科夫肝癌细胞核RNA中N6,O2-二甲基腺苷的特性分析

Characterization of N6, O2-dimethyladenosine from nuclear RNA of Novikoff hepatoma.

作者信息

Crain P F, Choi Y C, Busch H, McCloskey J A

出版信息

Nucleic Acids Res. 1978 Mar;5(3):771-6. doi: 10.1093/nar/5.3.771.

DOI:10.1093/nar/5.3.771
PMID:205837
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342022/
Abstract

A modified nucleoside was isolated from low molecular weight nuclear RNA of Novikoff hepatoma, the nucleotide sequence of which was reported earlier. The structure of this novel nucleoside was shown to be N6, O2'-dimethyladenosine (m6Am) by mass spectrometry of its trimethylsilyl derivative and by comparison with the mass spectra of N6- and O-2' monmethyl model compounds. The complete characterization was carried out using 0.04 A260 units (2 microgram) of m6Am.

摘要

从诺维科夫肝癌的低分子量核RNA中分离出一种修饰核苷，其核苷酸序列已于此前报道。通过对其三甲基硅烷基衍生物进行质谱分析，并与N6-和O-2'单甲基模型化合物的质谱进行比较，表明这种新型核苷的结构为N6，O2'-二甲基腺苷（m6Am）。使用0.04 A260单位（2微克）的m6Am进行了完整的表征。

相似文献

1
Characterization of N6, O2-dimethyladenosine from nuclear RNA of Novikoff hepatoma.诺维科夫肝癌细胞核RNA中N6,O2-二甲基腺苷的特性分析
Nucleic Acids Res. 1978 Mar;5(3):771-6. doi: 10.1093/nar/5.3.771.
2
Localization and phosphorylation of nuclear, nucleolar and extranucleolar non-histone proteins of Novikoff hepatoma ascites cells.诺维科夫肝癌腹水细胞的细胞核、核仁及核仁外非组蛋白的定位与磷酸化作用
J Mol Biol. 1975 Oct 5;97(4):611-9. doi: 10.1016/s0022-2836(75)80062-3.
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Primary sequence of U-1 nuclear ribonucleic acid of Novikoff hepatoma ascites cells.诺维科夫肝癌腹水细胞U-1核糖核酸的一级序列。
J Biol Chem. 1974 Oct 25;249(20):6486-94.
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[Comparative study of the low-molecular nuclear RNAs in the rat and in Seidel's hepatoma].[大鼠与赛德尔肝癌中低分子核RNA的比较研究]
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Identification of methylated nucleosides in messenger RNA from Novikoff hepatoma cells.诺维科夫肝癌细胞信使核糖核酸中甲基化核苷的鉴定
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Characterization of Novikoff hepatoma mRNA methylation and heterogeneity in the methylated 5' terminus.诺维科夫肝癌mRNA甲基化及甲基化5'末端异质性的表征
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Characterization of nuclear and chromatin poly A-containing RNA.细胞核和染色质中含多聚腺苷酸RNA的特性分析
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引用本文的文献

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Dynamic regulation and key roles of ribonucleic acid methylation.核糖核酸甲基化的动态调控及关键作用
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Summary: the modified nucleosides of RNA.总结：RNA的修饰核苷。
Nucleic Acids Res. 1994 Jun 25;22(12):2183-96. doi: 10.1093/nar/22.12.2183.

本文引用的文献

1
Occurrence of N2,N2,7-trimethylguanosine in minor RNA species of a mammalian cell line.N2,N2,7-三甲基鸟苷在一种哺乳动物细胞系的次要RNA种类中的出现情况。
Nature. 1969 Sep 27;223(5213):1365-6. doi: 10.1038/2231365a0.
2
Mass spectrometry of nucleic acid components. Trimethylsilyl derivatives of nucleotides, nucleosides, and bases.核酸成分的质谱分析。核苷酸、核苷和碱基的三甲基硅烷基衍生物。
J Am Chem Soc. 1968 Jul 17;90(15):4182-4. doi: 10.1021/ja01017a062.
3
Mass spectrometry of nucleic acid components. Analogs of adenosine.核酸成分的质谱分析。腺苷类似物。
J Am Chem Soc. 1970 Apr 22;92(8):2510-22. doi: 10.1021/ja00711a049.
4
Mass spectrometry of 7-methylpurine nucleosides: studies of a characteristic oxygen incorporation reaction that occurs during trimethylsilylation.7-甲基嘌呤核苷的质谱分析：对三甲基硅烷化过程中发生的特征性氧掺入反应的研究。
Anal Biochem. 1972 Nov;50(1):110-21. doi: 10.1016/0003-2697(72)90491-5.
5
Isolation and characterization of N6-methyladenosine from Escherichia coli valine transfer RNA.从大肠杆菌缬氨酸转移核糖核酸中分离和鉴定N6-甲基腺苷
Biochim Biophys Acta. 1969 Oct 22;190(2):264-73. doi: 10.1016/0005-2787(69)90078-1.
6
Mass spectrometry of nucleic acid components. N,O-Permethyl derivatives of nucleosides.核酸成分的质谱分析。核苷的N,O-二甲基衍生物。
J Am Chem Soc. 1973 Oct 31;95(22):7480-90. doi: 10.1021/ja00803a044.
7
Structure-retention relationships in the gas chromatography of nucleosides.核苷气相色谱中的结构-保留关系
Anal Chem. 1974 Sep;46(11):1378-83. doi: 10.1021/ac60347a033.
8
Primary sequence of U-1 nuclear ribonucleic acid of Novikoff hepatoma ascites cells.诺维科夫肝癌腹水细胞U-1核糖核酸的一级序列。
J Biol Chem. 1974 Oct 25;249(20):6486-94.
9
MOdified nucleosides of nuclear and nucleolar low molecular weight ribonucleic acid.细胞核和核仁低分子量核糖核酸的修饰核苷
J Biol Chem. 1972 Nov 25;247(22):7245-50.
10
N6, O2'-dimethyladenosine a novel methylated ribonucleoside next to the 5' terminal of animal cell and virus mRNAs.N6, O2'-二甲基腺苷，一种位于动物细胞和病毒mRNA 5'末端相邻位置的新型甲基化核糖核苷。
Nature. 1975 Sep 18;257(5523):251-3. doi: 10.1038/257251a0.