Volsky D J, Loyter A
Biochim Biophys Acta. 1978 Dec 19;514(2):213-24. doi: 10.1016/0005-2736(78)90293-6.
Chicken erythrocytes were fused either by Sendai virus or by the combination of Ca2+ and ionophore A23187. Intramembrane particles and external anionic sites of cells undergoing fusion were found to acquire the ability to undergo a process of cold-induced clustering (thermotropic separation). Cationized ferritin (200 microgram/ml 5% (v/v) cell suspension) inhibited both the fusion process and the thermotropic separation of intramembrane particles and external anionic sites. The correlation between the mobility of membrane proteins and the fusion process is discussed. It is suggest that an increase in the lateral mobility of membrane proteins is a prerequisite for initiation of membrane fusion.
鸡红细胞通过仙台病毒或Ca2+与离子载体A23187的组合进行融合。发现正在融合的细胞的膜内颗粒和外部阴离子位点获得了经历冷诱导聚集（热致分离）过程的能力。阳离子化铁蛋白（200微克/毫升5%（v/v）细胞悬液）抑制融合过程以及膜内颗粒和外部阴离子位点的热致分离。讨论了膜蛋白流动性与融合过程之间的相关性。有人提出，膜蛋白横向流动性的增加是膜融合起始的先决条件。
