Langness U, Löwensprung E
Res Exp Med (Berl). 1978 Mar 20;172(2):193-201. doi: 10.1007/BF01851411.
L 929 mouse fibroblasts were cultured as monolayer using MEM Eagle medium after addition of bovine serum albumin, antibiotics, antimycotica, ferrous nitrate and ascorbic acid. In 24 hours distances cell number was counted. At the same time peptidyl prolyl hydroxylase activity was determined and related to the protein concentration in the cells. The proliferation of the cells followed a typical curve. The cell number decreased after trypsination, then increased logarithmically, finally reached a stationary phase. Enzyme activity showed a correspondent curve. Active enzyme did not appear until near the end of the log phase. After addition of the mesenchyme active substances in therapeutical concentrations proliferation and synthesis of active peptidyl prolyl hydroxylase in the cells were significantly decreased. The results are discussed in relation to the characteristics of collagen synthesis and proliferation of connective tissue cells under physiological, pathophysiological and therapeutical conditions.
在添加牛血清白蛋白、抗生素、抗真菌剂、硝酸亚铁和抗坏血酸后，使用伊格尔MEM培养基将L929小鼠成纤维细胞培养成单层。在24小时的时间间隔内对细胞数量进行计数。同时测定肽基脯氨酰羟化酶活性，并将其与细胞中的蛋白质浓度相关联。细胞增殖遵循典型曲线。胰蛋白酶消化后细胞数量减少，然后呈对数增加，最终达到稳定期。酶活性呈现相应曲线。活性酶直到对数期接近结束时才出现。添加治疗浓度的间充质活性物质后，细胞中活性肽基脯氨酰羟化酶的增殖和合成显著降低。结合生理、病理生理和治疗条件下胶原蛋白合成和结缔组织细胞增殖的特点对结果进行了讨论。
