Department of Surgery, Campus Charité Mitte and Campus Virchow-Klinikum, Charité - Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany.
Eur J Med Res. 2021 Jan 9;26(1):6. doi: 10.1186/s40001-020-00478-3.
Rodent models of liver resection have been used to investigate and evaluate the liver's complex physiology and pathology since 1931. First documented by Higgins and Anderson, such models were created to understand liver regeneration mechanisms to improve outcomes in patients undergoing extensive liver resection for liver cancer or other underlying liver diseases.
A systematic search was conducted using Pubmed, gathering publications up to January 2019, which engaged with the mouse model of extended liver resection as a method itself. The results of this search were filtered according to their language, novelty, and relevancy.
The Boolean search found 3741 articles on Pubmed, with 3130 publications remaining when filtered by language and the presence of a full text. In total, 21 of these publications examined the key themes of the animal model described. The mortality varied from 0 to 50% depending on the surgeon's experience and the resection method. The liver resection was mainly performed with classic sutures (14 out of 21 publications) and isoflurane was used for anaesthesia (10 out of 21 publications) in combination with analgesics (buprenorphine or ketamine/xylazine). The most used mouse strain was C57BL/6 (7 of 21 publications) which was on average 11 weeks old with a weight of 23 g.
Through the overview, laid out in the selected publications, this paper reviews the shift of the extended liver resection model from rat to the mouse, describes the state of the art in the experimental setting, and discusses the possible limitations and pitfalls. Clearly, the extended liver resection in mice is a reproducible, practical and easy to learn method.
自 1931 年以来，啮齿动物肝切除术模型一直被用于研究和评估肝脏的复杂生理学和病理学。该模型由 Higgins 和 Anderson 首次记录，旨在了解肝脏再生机制，以改善因肝癌或其他潜在肝脏疾病而接受广泛肝切除术的患者的预后。
通过 Pubmed 进行了系统搜索，收集了截至 2019 年 1 月的出版物，这些出版物涉及将延长肝切除术的小鼠模型作为一种方法本身。根据语言、新颖性和相关性对搜索结果进行了过滤。
在 Pubmed 上进行布尔搜索找到了 3741 篇文章，通过语言和全文的存在过滤后，剩下 3130 篇出版物。总共有 21 篇出版物检查了所描述的动物模型的关键主题。根据外科医生的经验和切除方法，死亡率从 0%到 50%不等。肝切除术主要采用经典缝线（21 篇出版物中有 14 篇），麻醉采用异氟烷（21 篇出版物中有 10 篇）并结合使用镇痛药（丁丙诺啡或氯胺酮/甲苯噻嗪）。使用最多的小鼠品系是 C57BL/6（21 篇出版物中有 7 篇），平均年龄为 11 周，体重为 23 克。
通过对选定出版物的概述，本文回顾了从大鼠到小鼠的延长肝切除术模型的转变，描述了实验设置的最新技术，并讨论了可能存在的局限性和陷阱。显然，小鼠的延长肝切除术是一种可重复、实用且易于学习的方法。
