Thavarungkul P, Håkanson H, Holst O, Mattiasson B
Department of Biotechnology, University of Lund, Sweden.
Biosens Bioelectron. 1991;6(2):101-7. doi: 10.1016/0956-5663(91)87033-8.
Urease was immobilized to porous glass and used in combination with a conductivity meter for determining urea in standard solutions as well as in blood from a patient undergoing dialysis. The sampling unit involves a possibility for heparinization at the sampling point and a dialysis step prior to exposure to the enzyme column. The unit operates in a linear mode in the concentration range 5-50 mM. Monitoring of dialysis process gave good correlation with off-line analyses.
脲酶被固定在多孔玻璃上，并与电导率仪结合使用，用于测定标准溶液以及正在接受透析的患者血液中的尿素。采样单元包括在采样点进行肝素化处理的可能性以及在接触酶柱之前的透析步骤。该单元在5-50 mM的浓度范围内呈线性模式运行。透析过程的监测与离线分析具有良好的相关性。
