W. Harry Feinstone Department of Molecular Microbiology and Immunology, The Johns Hopkins Malaria Research Institute, Baltimore, MD, USA.
Am J Trop Med Hyg. 2010 Jul;83(1):33-7. doi: 10.4269/ajtmh.2010.09-0296.
Anopheles arabiensis is a major vector of Plasmodium falciparum in southern Zambia. This study aimed to determine the rate of multiple human blood meals taken by An. arabiensis to more accurately estimate entomologic inoculation rates (EIRs). Mosquitoes were collected in four village areas over two seasons. DNA from human blood meals was extracted and amplified at four microsatellite loci. Using the three-allele method, which counts > or = 3 alleles at any microsatellite locus as a multiple blood meal, we determined that the overall frequency of multiple blood meals was 18.9%, which was higher than rates reported for An. gambiae in Kenya and An. funestus in Tanzania. Computer simulations showed that the three-allele method underestimates the true multiple blood meal proportion by 3-5%. Although P. falciparum infection status was not shown to influence the frequency of multiple blood feeding, the high multiple feeding rate found in this study increased predicted malaria risk by increasing EIR.
在赞比亚南部，阿拉伯按蚊是恶性疟原虫的主要传播媒介。本研究旨在确定阿拉伯按蚊吸食多人血的比率，以便更准确地估计昆虫接种率（EIR）。在两个季节里，于四个村庄地区采集了蚊子。从人血餐中提取 DNA，并在四个微卫星基因座上进行扩增。使用三等位基因法（任何微卫星基因座上的 > = 3 个等位基因计为多个人血餐），我们确定多个人血餐的总体频率为 18.9%，高于肯尼亚的冈比亚按蚊和坦桑尼亚的致倦库蚊的报告率。计算机模拟表明，三等位基因法低估了真实的多人血餐比例 3-5%。尽管疟原虫感染状况并未显示影响多人血餐的频率，但本研究中发现的高多人血餐率通过增加 EIR 增加了疟疾风险的预测。
