De Benedictis John, Chow-Shaffer Esther, Costero Adriana, Clark Gary G, Edman John D, Scott Thomas W
Department of Entomology, University of California, Davis, California 95616, USA.
Am J Trop Med Hyg. 2003 Apr;68(4):437-46.
We used polymerase chain reaction-based DNA profiling to construct allelic profiles for residents and visitors of 22 houses in Florida, Puerto Rico, and human DNA from blood meals in Aedes aegypti that were collected in those homes. Complete profiles were obtained for < or = 2 days after blood ingestion. Eighteen percent of the meals came from two different people. There was no evidence of meals from > or = 2 people. Eighty percent of the meal sources were identified, > 70% were taken from residents of the collection house, and > 90% were from residents of the study community. Across the community, feeding was non-random with a bias towards young adults and males. Three people accounted for 56% of the meals. Our results confirm that multiple feeding on different people is an important component in the role of Ae. aegypti in dengue virus transmission and help explain the spatial distribution of dengue cases in a previous epidemic in Florida, Puerto Rico.
我们使用基于聚合酶链反应的DNA分析方法，为佛罗里达州、波多黎各22所房屋的居民和访客构建等位基因图谱，并对在这些房屋中采集的埃及伊蚊血餐中的人类DNA进行分析。吸血后≤2天即可获得完整图谱。18%的血餐来自两个不同的人。没有证据表明血餐来自≥2个人。80%的血餐来源得到了确定，其中>70%取自采集房屋的居民，>90%来自研究社区的居民。在整个社区中，吸血具有非随机性，偏向于年轻人和男性。三个人占了56%的血餐。我们的结果证实，埃及伊蚊对不同人进行多次吸血是其在登革热病毒传播中发挥作用的一个重要因素，并有助于解释佛罗里达州、波多黎各先前一次疫情中登革热病例的空间分布情况。
