Department of Biology, Jimma University, Jimma, Ethiopia.
Am J Trop Med Hyg. 2010 Jul;83(1):122-5. doi: 10.4269/ajtmh.2010.09-0738.
The status of knockdown resistance (kdr) mutation was investigated in the major malaria vector Anopheles arabiensis Patton (Diptera: Culicidae) from Ethiopia. Among 240 mosquito samples from 15 villages of southwestern Ethiopia that were screened by allele-specific polymerase chain reaction for kdr mutations, the West African kdr mutation (L1014F) was detected in almost all specimens (98.5%), whereas the East African kdr mutation (L1014S) was absent. Moreover, the mortality of An. gambiae s.l. to diagnostic dosages of 4% DDT, 0.75% permethrin, and 0.05% deltamethrin from bioassay results was 1.0%, 18.1%, and 82.2%, respectively. We report here the highest kdr allele frequency ever observed in An. arabiensis and its implications in malaria vector control in Ethiopia are discussed.
我们调查了来自埃塞俄比亚的主要疟疾传播媒介按蚊属埃及伊蚊（双翅目：蚊科）的击倒抗性（kdr）突变体的状态。在通过等位基因特异性聚合酶链反应筛选来自埃塞俄比亚西南部 15 个村庄的 240 个蚊子样本中，几乎所有标本（98.5%）都检测到了西非 kdr 突变（L1014F），而东非 kdr 突变（L1014S）则不存在。此外，按蚊属的死亡率。从生物测定结果来看，对 4%滴滴涕、0.75%氯菊酯和 0.05%溴氰菊酯的诊断剂量分别为 1.0%、18.1%和 82.2%。我们在这里报告了在按蚊属埃及伊蚊中观察到的最高 kdr 等位基因频率，并讨论了其对埃塞俄比亚疟疾媒介控制的影响。
