Department of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Zhejiang University, Hangzhou 310027, China.
Biomaterials. 2010 Oct;31(29):7534-41. doi: 10.1016/j.biomaterials.2010.06.019. Epub 2010 Jul 2.
A new complex multiple forklike ZnO nanostructure was fabricated by citric acid assisted annealing process. The complex multiple forklike ZnO nanostructure is composed of several nanorods. These nanorods grow from a thin platelet base and are parallel to each other to form complex multiple forklike ZnO nanostructure. The widthes of thin platelet bases range from 60 to 230 nm. The diameters of the nanorods range from 16 to 60 nm, and their lengths are 0.5 mu approximately 1.3mu. FT-IR spectrum, room-temperature PL, and UV-Vis absorption spectra are also discussed. A possible growth mechanism is proposed. Complex multiple forklike ZnO nanostructure was first used to construct a novel enzymatic biosensor based on the ZnO/CHIT inorganic-organic composite film. Well-studied horseradish peroxidase (HRP) was chosen as a model enzyme. The as-prepared biosensor displays ultrahigh sensitivity, quick response time, low detection limit and small apparent Michaelis-Menten constant. These results show that the Complex multiple forklike ZnO nanostructure is a promising material to construct enzyme biosensors.
采用柠檬酸辅助退火工艺制备了一种新的复杂的多叉状 ZnO 纳米结构。该复杂的多叉状 ZnO 纳米结构由几个纳米棒组成。这些纳米棒从一个薄的板状基底生长，彼此平行排列，形成复杂的多叉状 ZnO 纳米结构。薄的板状基底的宽度范围从 60nm 到 230nm。纳米棒的直径范围从 16nm 到 60nm，长度约为 0.5μm 到 1.3μm。还讨论了傅里叶变换红外光谱（FT-IR）、室温光致发光（PL）和紫外-可见吸收光谱。提出了一种可能的生长机制。首次将复杂的多叉状 ZnO 纳米结构用于构建基于 ZnO/CHIT 无机-有机复合膜的新型酶生物传感器。选择了研究充分的辣根过氧化物酶（HRP）作为模型酶。所制备的生物传感器具有超高的灵敏度、快速的响应时间、低的检测限和小的表观米氏常数。这些结果表明，复杂的多叉状 ZnO 纳米结构是构建酶生物传感器的一种很有前途的材料。
