State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China.
Anal Biochem. 2010 Oct 15;405(2):207-12. doi: 10.1016/j.ab.2010.06.022. Epub 2010 Jun 17.
Multifunctional poly(glycidyl methacrylate) (PGMA) microspheres containing magnetic, fluorescent, and cancer cell-specific moieties were prepared in four steps: (i) preparation of parent PGMA microspheres by dispersion polymerization and their reaction with ethylenediamine to obtain amino groups, (ii) precipitation of iron ions (Fe(2+) and Fe(3+)) to form Fe(3)O(4) nanoparticles within the microspheres, (iii) consecutive reactions of folic acid with the amino groups on PGMA, and (iv) incorporation of fluorescein isothiocyanate into the microspheres. The microspheres were superparamagnetic, highly monodispersive, intensively fluorescent, and capable of recognizing and binding cancer cells that overexpress folic acid receptors. It was demonstrated that with these microspheres, HeLa cells could be captured from their suspension and easily moved in the direction of the externally applied magnetic field.
多功能聚（甲基丙烯酸缩水甘油酯）（PGMA）微球含有磁性、荧光和癌细胞特异性部分，通过以下四个步骤制备：（i）通过分散聚合制备母体 PGMA 微球，并与乙二胺反应以获得氨基，（ii）沉淀铁离子（Fe(2+) 和 Fe(3+)）以在微球内形成 Fe(3)O(4)纳米粒子，（iii）叶酸与 PGMA 上的氨基连续反应，（iv）将异硫氰酸荧光素掺入微球中。微球具有超顺磁性、高度单分散性、强荧光性，并且能够识别和结合过度表达叶酸受体的癌细胞。结果表明，使用这些微球可以从悬液中捕获 HeLa 细胞，并在外加磁场的作用下轻松移动。
