Program for the Biology of Filamentous Fungi, Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, USA.
Fungal Genet Biol. 2010 Oct;47(10):789-99. doi: 10.1016/j.fgb.2010.06.007. Epub 2010 Jun 26.
Striatin family proteins have been identified in animals and fungi and are considered to be scaffolding proteins. In fungi striatin orthologs have been associated with sexual development and virulence to plants. In this study, we characterized the functions and localization of the striatin ortholog, StrA, in Aspergillus nidulans. deltastrA strains showed multiple defects in conidium germination, mycelial radial growth, production of diffusible red pigment, and reduced conidiation. The most striking phenotype is the production of abnormally small cleistothecia that are defective in ascosporogenesis. Over-expression of strA enhanced cleistothecium development and increased the production of Hülle cells in shaking liquid cultures. In addition, we generated strains expressing StrA::eGFP under the endogenous promoter. By co-labeling with FM4-64 and co-localization with nuclear localized StuA(NLS)::DsRed or CxnA (an endoplasmic reticulum marker), we determined that StrA mainly localizes to endoplasmic reticulum and the nuclear envelope.
Striatin 家族蛋白在动物和真菌中被鉴定出来，被认为是支架蛋白。在真菌中，striatin 同源物与有性发育和对植物的毒性有关。在这项研究中，我们描述了 Aspergillus nidulans 中 striatin 同源物 StrA 的功能和定位。ΔstrA 菌株在分生孢子萌发、菌丝径向生长、扩散红色色素的产生和产孢能力方面表现出多种缺陷。最显著的表型是产生异常小的闭囊壳，这些闭囊壳在减数分裂过程中存在缺陷。StrA 的过表达增强了闭囊壳的发育，并增加了振荡液体培养中 Hülle 细胞的产生。此外，我们生成了在内源启动子下表达 StrA::eGFP 的菌株。通过与 FM4-64 共标记，并与核定位的 StuA(NLS)::DsRed 或 CxnA（内质网标记物）共定位，我们确定 StrA 主要定位于内质网和核膜。
