Department of Marine Biotechnology, National Kaohsiung Marine University, 142 Hai-Chuan Road, Nan-Tzu, Kaohsiung City 811, Taiwan ROC.
College of Agriculture and Natural Resources, Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 402, Taiwan ROC.
Int J Syst Evol Microbiol. 2011 Jun;61(Pt 6):1402-1407. doi: 10.1099/ijs.0.025403-0. Epub 2010 Jul 2.
A bacterial strain, designated an-8(T), was isolated from a freshwater shrimp culture pond in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain an-8(T) were Gram-reaction-negative, aerobic, rod-shaped and non-motile, formed yellow-pigmented colonies and grew at 15-30 °C (optimum 25 °C), pH 7-8 (optimum pH 8.0) and in 0-1 % (w/v) NaCl (optimum 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain an-8(T) belonged to the genus Flavobacterium and its most closely related neighbours were Flavobacterium terrigena DS-20(T) and Flavobacterium terrae R2A1-13(T) with sequence similarities of 95.1 and 94.9 %, respectively. Strain an-8(T) contained iso-C(15 : 0), summed feature 3 (C(16 : 1)ω6c and/or C(16 : 1)ω7c), iso-C(16 : 0) 3-OH, iso-C(15 : 0) 3-OH, iso-C(17 : 0) 3-OH and iso-C(15 : 1) as the major fatty acids. The major isoprenoid quinone was MK-6. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidyldimethylethanolamine, phosphatidylserine and several unidentified polar lipids. The G+C content of the genomic DNA was 39.8 mol%. On the basis of the phylogenetic and phenotypic data, strain an-8(T) represents a novel species of the genus Flavobacterium, for which the name Flavobacterium macrobrachii sp. nov. is proposed. The type strain is an-8(T) ( = BCRC 17965(T) = LMG 25203(T)).
一株细菌菌株，命名为 an-8(T)，从台湾的淡水虾养殖池塘中分离出来，并通过多相分类学方法进行了特征描述。菌株 an-8(T)的细胞革兰氏反应阴性，需氧，杆状，不运动，形成黄色色素菌落，在 15-30°C（最佳 25°C）、pH 7-8（最佳 pH 8.0）和 0-1%（w/v）NaCl（最佳 0%）中生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 an-8(T)属于黄杆菌属，与最接近的近缘种 Flavobacterium terrigena DS-20(T)和 Flavobacterium terrae R2A1-13(T)的序列相似性分别为 95.1%和 94.9%。菌株 an-8(T)含有 iso-C(15:0)、summed feature 3 (C(16:1)ω6c 和/或 C(16:1)ω7c)、iso-C(16:0) 3-OH、iso-C(15:0) 3-OH、iso-C(17:0) 3-OH 和 iso-C(15:1) 作为主要脂肪酸。主要的异戊二烯醌是 MK-6。极性脂类谱由磷脂酰乙醇胺、磷脂酰二甲乙醇胺、磷脂酰丝氨酸和几种未鉴定的极性脂类组成。基因组 DNA 的 G+C 含量为 39.8 mol%。基于系统发育和表型数据，菌株 an-8(T)代表黄杆菌属的一个新种，建议将其命名为 Flavobacterium macrobrachii sp. nov.。模式菌株为 an-8(T)（=BCRC 17965(T)=LMG 25203(T)）。
