Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Int J Syst Evol Microbiol. 2012 May;62(Pt 5):1048-1055. doi: 10.1099/ijs.0.034884-0. Epub 2011 Jun 17.
A yellow-pigmented bacterial strain designated TNR-25(T) was isolated from spring water in Taiwan and was characterized using a polyphasic taxonomic approach. Strain TNR-25(T) was gram-negative, obligately aerobic, rod-shaped, non-motile and non-spore-forming. Growth occurred at 15-40 °C (optimum, 25 °C), at pH 6.0-10.0 (optimum, pH 7.0) and with 0-0.5 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TNR-25(T) belonged to the genus Inhella and its closest neighbour was Inhella inkyongensis IMCC1713(T) with 98.1 % sequence similarity. The major fatty acids (>10 %) of strain TNR-25(T) were summed feature 3 (comprising C(16 : 1)ω7c and/or C(16 : 1)ω6c) and C(16 : 0). The major cellular hydroxy fatty acids were C(10 : 0) 3-OH and C(12 : 0) 3-OH. The isoprenoid quinone was Q-8 and the DNA G+C content was 69.6 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, diphosphatidylglycerol and several uncharacterized phospholipids. The DNA-DNA relatedness between strain TNR-25(T) and I. inkyongensis IMCC1713(T) was about 30.6-35.5 %. On the basis of the genotypic and phenotypic data, strain TNR-25(T) represents a novel species in the genus Inhella, for which the name Inhella fonticola sp. nov. is proposed; the type strain is TNR-25(T) ( = BCRC 80211(T) = LMG 25721(T)).
一株黄色着色的细菌菌株命名为 TNR-25(T)，从台湾泉水分离得到，并通过多相分类学方法进行了特征描述。菌株 TNR-25(T) 为革兰氏阴性，严格需氧，杆状，无运动性，不形成孢子。在 15-40°C（最佳，25°C），pH 6.0-10.0（最佳，pH 7.0）和 0-0.5%NaCl（最佳，0%）的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 TNR-25(T) 属于 Inhella 属，与其最接近的是 Inhella inkyongensis IMCC1713(T)，序列相似度为 98.1%。菌株 TNR-25(T) 的主要脂肪酸（>10%）为(summed feature 3，包括 C(16:1)ω7c 和/或 C(16:1)ω6c) 和 C(16:0)。主要的细胞羟基脂肪酸为 C(10:0) 3-OH 和 C(12:0) 3-OH。异戊烯醌为 Q-8，DNA G+C 含量为 69.6 mol%。极性脂谱由磷脂酰乙醇胺、磷脂酰甘油、磷脂酰丝氨酸、双磷脂酰甘油和几种未鉴定的磷脂组成。菌株 TNR-25(T) 和 I. inkyongensis IMCC1713(T) 之间的 DNA-DNA 同源性约为 30.6-35.5%。基于基因型和表型数据，菌株 TNR-25(T) 代表 Inhella 属中的一个新种，命名为 Inhella fonticola sp. nov.；模式菌株为 TNR-25(T)（=BCRC 80211(T)=LMG 25721(T)）。
