Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University, 1 Seocheon, Kihung Yongin, Kyunggi 449-701, Republic of Korea.
Int J Syst Evol Microbiol. 2011 Jun;61(Pt 6):1408-1412. doi: 10.1099/ijs.0.025700-0. Epub 2010 Jul 2.
A Gram-negative, aerobic, non-motile, non-spore-forming, yellow-pigmented, rod-shaped bacterium, designated strain THG 01(T), was isolated from the soil of a ginseng field in Pocheon province, South Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain THG 01(T) grew well at 25-37 °C and pH 6.0-7.5 in the absence of NaCl on nutrient agar. On the basis of 16S rRNA gene sequence similarity data, strain THG 01(T) was shown to belong to the family Flavobacteriaceae and was related to Flavobacterium anhuiense D3(T) (97.5 % similarity), Flavobacterium johnsoniae UW101(T) (96.8 %) and Flavobacterium denitrificans ED5(T) (96.7 %). 16S rRNA gene sequence similarities between the novel strain and members of other recognized species within the family Flavobacteriaceae were less than 96.7 %. The G+C content of the genomic DNA of strain THG 01(T) was 32.1 mol%. Phenotypic and chemotaxonomic data (major menaquinone was MK-6 and major fatty acids were iso-C(15 : 0), iso-C(15 : 0) 3-OH and C(16 : 1)ω7c and/or C(16 : 1)ω6c ) supported the affiliation of strain THG 01(T) to the genus Flavobacterium. DNA-DNA hybridization experiments showed that DNA-DNA relatedness values between strain THG 01(T) and its closest phylogenetic neighbours were below 11 %. The results of physiological and biochemical tests enabled strain THG 01(T) to be differentiated genotypically and phenotypically from recognized species of the genus Flavobacterium. The isolate therefore represents a novel species, for which the name Flavobacterium ginsenosidimutans sp. nov. is proposed, with THG 01(T) ( = KACC 14525(T) = JCM 16720(T)) as the type strain.
一株革兰氏阴性、需氧、非运动、非孢子形成、黄色素产生、杆状细菌，被命名为 THG 01(T) 菌株，从韩国抱川省人参田的土壤中分离得到，并通过多相研究方法研究了其分类地位。THG 01(T) 菌株在无 NaCl 的营养琼脂上，在 25-37°C 和 pH6.0-7.5 的条件下生长良好。根据 16S rRNA 基因序列相似性数据，THG 01(T) 菌株属于黄杆菌科，与黄杆菌属的安华黄杆菌 D3(T)（97.5%相似性）、约翰逊黄杆菌 UW101(T)（96.8%相似性）和脱氮黄杆菌 ED5(T)（96.7%相似性）最为相似。该新菌株与黄杆菌科内其他公认种属的成员之间的 16S rRNA 基因序列相似性均小于 96.7%。THG 01(T) 菌株的基因组 DNA G+C 含量为 32.1mol%。表型和化学分类学数据（主要的menaquinone 是 MK-6，主要的脂肪酸是 iso-C(15:0)、iso-C(15:0)3-OH 和 C(16:1)ω7c 和/或 C(16:1)ω6c）支持 THG 01(T) 菌株属于黄杆菌属。DNA-DNA 杂交实验表明，THG 01(T) 菌株与其最接近的系统发育近邻之间的 DNA-DNA 同源性值低于 11%。生理生化测试的结果使 THG 01(T) 菌株在基因型和表型上与黄杆菌属的公认种属区分开来。因此，该分离株代表了一个新的种属，命名为人参黄杆菌 Flavobacterium ginsenosidimutans sp. nov.，其模式菌株为 THG 01(T)（ = KACC 14525(T) = JCM 16720(T)）。
