Kim Sang-Rae, Kim Yeon-Ju, Nguyen Ngoc-Lan, Min Jin-Woo, Jeon Ji-Na, Yang Dong-Uk, Yang Deok-Chun
Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University, 1 Seocheon-dong, Gyeonggi-do, South Korea.
J Gen Appl Microbiol. 2011;57(6):341-6. doi: 10.2323/jgam.57.341.
A novel strain of Flavobacterium, DCY55(T), a Gram-negative, yellow-pigmented, rod-shaped, non-spore-forming and gliding-motile bacterium, was isolated from the soil of a ginseng field in South Korea. Phylogenetic analysis, based on the 16S rRNA sequence, demonstrated that strain DCY55(T) belongs to the genus Flavobacterium within the family Flavobacteriaceae. Strain DCY55(T) showed the highest similarity with F. johnsoniae UW101(T) (97.1%), F. ginsenosidimutans THG 01(T) (96.8%), F. defluvii EMB 117(T) (96.6%), F. banpakuense 15F3(T) (96.3%) and F. anhuiense D3(T) (95.8%). Chemotaxonomic results showed that strain DCY55(T) predominantly contains menaquinone MK-6, that its DNA G+C content is 36.1mol%, and that its major cellular fatty acids are iso-C(15:0), summed feature 3 (comprising iso-C(15:0) 2-OH and/or C(16:1) ω 7c) and C(16:0). The chemotaxonomic and genotypic characteristics support the taxonomic classification of strain DCY55(T) to the genus Flavobacterium. The results of physiological and biochemical tests confirmed that strain DCY55(T) is distinct from previously validated species. We conclude that strain DCY55(T) should be classified as a novel species of the genus Flavobacterium, for which the name Flavobacterium ginsengiterrae sp. nov. is proposed, with the type strain DCY55(T) (=KCTC 23319(T) = JCM 17337(T)).
从韩国人参田土壤中分离出一种新型黄杆菌菌株DCY55(T)，它是革兰氏阴性、黄色色素沉着、杆状、不形成芽孢且能滑行运动的细菌。基于16S rRNA序列的系统发育分析表明，菌株DCY55(T)属于黄杆菌科黄杆菌属。菌株DCY55(T)与琼氏黄杆菌UW101(T)（97.1%）、人参皂苷转化黄杆菌THG 01(T)（96.8%）、污水黄杆菌EMB 117(T)（96.6%）、斑帕克黄杆菌15F3(T)（96.3%）和安徽黄杆菌D3(T)（95.8%）的相似性最高。化学分类学结果表明，菌株DCY55(T)主要含有甲基萘醌MK-6，其DNA G+C含量为36.1mol%，主要细胞脂肪酸为异-C(15:0)、特征性总和3（包括异-C(15:0) 2-OH和/或C(16:1) ω 7c）和C(16:0)。化学分类学和基因型特征支持将菌株DCY55(T)分类到黄杆菌属。生理生化测试结果证实，菌株DCY55(T)与先前已验证的物种不同。我们得出结论，菌株DCY55(T)应被分类为黄杆菌属的一个新物种，为此提出名称人参土黄杆菌（Flavobacterium ginsengiterrae sp. nov.），模式菌株为DCY55(T)（=KCTC 23319(T) = JCM 17337(T)）。
