BioFil Microbiological, Biotechnological and Biochemical Ltd, Budapest, Hungary.
Research Group for Food Biotechnology, Institute of Food Science and Technology, Hungarian University of Agriculture and Life Sciences, Budapest, Hungary.
Arch Microbiol. 2022 May 6;204(6):301. doi: 10.1007/s00203-022-02905-x.
A Gram-negative bacterial strain, named Kb82, was isolated from agricultural soil and a polyphasic approach was used for characterisation and to determine its taxonomic position. Based on 16S rRNA gene sequence analysis, the highest similarity was found with Flavobacterium artemisiae SYP-B1015 (98.2%). The highest ANI (83.3%) and dDDH (26.5%) values were found with Flavobacterium ginsenosidimutans THG 01 and Flavobacterium fluviale HYN0086, respectively. The isolate is aerobic with rod-shaped cells, positive for catalase and negative for oxidase tests. The DNA G+C content is 34.7 mol%. The only isoprenoid quinone is menaquinone 6 (MK-6). The major fatty acids are iso-C, summed feature 3 (C ω7c/C ω6c) and iso-C 3OH. The major polar lipid is phosphatidylethanolamine. On the bases of phenotypic characteristics and analysis of 16S rRNA gene sequences, it is concluded that strain Kb82 represents a novel species in the Flavobacterium genus, for which the name Flavobacterium hungaricum sp. nov. is proposed. The type strain of the species is strain Kb82 (= LMG 31576 = NCAIM B.02635).
一株革兰氏阴性细菌菌株 Kb82 从农业土壤中分离得到，采用多相分类方法对其进行了特征描述和分类地位的确定。基于 16S rRNA 基因序列分析，与 Flavobacterium artemisiae SYP-B1015 的相似度最高（98.2%）。与 Flavobacterium ginsenosidimutans THG 01 和 Flavobacterium fluviale HYN0086 的最高 ANI（83.3%）和 dDDH（26.5%）值。该分离株为需氧菌，细胞呈杆状，过氧化氢酶阳性，氧化酶阴性。DNA G+C 含量为 34.7 mol%。唯一的类异戊二烯醌是menaquinone 6（MK-6）。主要脂肪酸是 iso-C、summed feature 3（C ω7c/C ω6c）和 iso-C 3OH。主要极性脂是磷脂酰乙醇胺。根据表型特征和 16S rRNA 基因序列分析，认为菌株 Kb82 代表黄杆菌属的一个新种，命名为 Flavobacterium hungaricum sp. nov.。该种的模式菌株为 Kb82 菌株（= LMG 31576 = NCAIM B.02635）。
