Institute of Medicinal Plant Development, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 100193, China.
Sci China Life Sci. 2010 Jun;53(6):701-8. doi: 10.1007/s11427-010-4009-1. Epub 2010 Jul 3.
DNA barcoding is a rapidly developing frontier technology that is gaining worldwide attention. Here, seven regions (psbA-trnH, matK, ycf5, rpoC1, rbcL, ITS2, and ITS) with potential for use as DNA barcodes were tested for their ability to identify 300 samples of 192 species from 72 genera of the family Rutaceae. To evaluate each barcode's utility for species authentication, PCR amplification efficiency, genetic divergence, and barcoding gaps were assessed. We found that the ITS2 region exhibited the highest inter-specific divergence, and that this was significantly higher than the intra-specific variation in the "DNA barcoding gap" assessment and Wilcoxon two-sample tests. The ITS2 locus had the highest identification efficiency among all tested regions. In a previous study, we found that ITS2 was able to discriminate a wide range of plant taxa, and here we confirmed that ITS2 was also able to discriminate a number of closely related species. Therefore, we propose that ITS2 is a promising candidate barcode for plant species identification.
DNA 条形码是一项快速发展的前沿技术，正在引起全球关注。在这里，我们测试了七个具有潜在用途的区域（psbA-trnH、matK、ycf5、rpoC1、rbcL、ITS2 和 ITS），以鉴定来自芸香科 72 属 192 种的 300 个样本。为了评估每个条形码在物种鉴定方面的实用性，我们评估了 PCR 扩增效率、遗传分化和条形码间隙。我们发现 ITS2 区域表现出最高的种间差异，这明显高于“DNA 条形码间隙”评估和 Wilcoxon 两样本检验中的种内变异。在所有测试的区域中，ITS2 位点具有最高的鉴定效率。在之前的一项研究中，我们发现 ITS2 能够区分广泛的植物分类群，在这里我们证实 ITS2 也能够区分一些密切相关的物种。因此，我们提出 ITS2 是一种很有前途的植物物种鉴定候选条形码。
