Miñana M D, Felipo V, Cortés F, Grisolía S
Instituto de Investigaciones Citológicas, Centro Asociado del CSIC, Valencia, Spain.
FEBS Lett. 1991 Jun 17;284(1):60-2. doi: 10.1016/0014-5793(91)80761-q.
We have shown that inhibition of protein kinase C by 1-5-isoquinolinylsulfonyl-2-methylpiperazine, H7, induces differentiation and inhibits proliferation of Neuro 2a cells. We have now tested if H7 is able to inhibit proliferation of: 1) human tumor cell lines from tissues other than brain; and 2) primary cultured cells from several human brain tumors. H7 inhibits, in a dose-dependent manner, proliferation of all human tumor cell lines tested and of primary cultured cells from human brain tumors. These results indicate that inhibition of protein kinase C inhibits proliferation of tumoral cells, therefore, H7, and likely other inhibitors of protein kinase C, could be useful in the clinical treatment of brain (and probably other) tumors.
我们已经证明，1-5-异喹啉磺酰基-2-甲基哌嗪（H7）对蛋白激酶C的抑制作用可诱导Neuro 2a细胞分化并抑制其增殖。我们现在测试了H7是否能够抑制以下细胞的增殖：1）来自脑以外组织的人类肿瘤细胞系；2）来自几种人类脑肿瘤的原代培养细胞。H7以剂量依赖的方式抑制所有测试的人类肿瘤细胞系以及来自人类脑肿瘤的原代培养细胞的增殖。这些结果表明，抑制蛋白激酶C可抑制肿瘤细胞的增殖，因此，H7以及可能的其他蛋白激酶C抑制剂可能对脑（可能还有其他）肿瘤的临床治疗有用。
