Parodi M T, Varesio L, Tonini G P
Laboratory of Molecular Immunoregulation, NCI-FGRF, Frederick, MD.
FEBS Lett. 1990 Aug 20;269(1):4-6. doi: 10.1016/0014-5793(90)81104-v.
It has been proved that inhibition of protein kinase C by 1-(5-isoquinolinylsulfonyl)-1-methylpiperazine (H7) induces morphological differentiation in murine neuroblastoma (nb) cell. Here we report that H7 is also active on human nb cell lines. The human nb cell had originally neuroblast-like (N) or intermediate (I) morphology. N and I type are thought to represent different stages of neuroblastoma differentiation. Neurite outgrowth was observed in N and I type morphology treating the cells with 7, 14 or 28 microM of H7. The results confirm previous observations and show that inhibition of PKC by H7 also promotes neuronal differentiation in human cell line variants.
业已证明，1-(5-异喹啉磺酰基)-1-甲基哌嗪（H7）对蛋白激酶C的抑制作用可诱导鼠神经母细胞瘤（nb）细胞发生形态分化。在此我们报告，H7对人nb细胞系也有活性。人nb细胞最初具有神经母细胞样（N）或中间型（I）形态。N型和I型被认为代表神经母细胞瘤分化的不同阶段。在用7、14或28微摩尔的H7处理细胞时，在N型和I型形态中均观察到了神经突生长。这些结果证实了先前的观察结果，并表明H7对蛋白激酶C的抑制作用也促进了人细胞系变体中的神经元分化。
