Miñana M D, Felipo V, Grisolía S
Instituto de Investigaciones Citológicas de la Caja de Ahorros de Valencia, Spain.
FEBS Lett. 1989 Sep 11;255(1):184-6. doi: 10.1016/0014-5793(89)81087-7.
It is shown that 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H7), a specific inhibitor of protein kinase C, induces neuritogenesis in neuro 2a cells. The percentage of differentiated cells was 9%, 20%, 59% and 85% at 0, 17, 85 and 500 microM H7, respectively. The number of neurites cell increased 2-, 8- and 14-fold over the controls for 17, 85 and 500 microM H7, respectively. These results indicate that protein kinase C plays a key role in the control of differentiation of neural cells and that its specific inhibition may be of basic as well as of practical importance.
结果表明，蛋白激酶C的特异性抑制剂1-(5-异喹啉磺酰基)-2-甲基哌嗪（H7）可诱导神经2a细胞发生神经突生长。在0、17、85和500微摩尔/升的H7浓度下，分化细胞的百分比分别为9%、20%、59%和85%。对于17、85和500微摩尔/升的H7，神经突细胞数量分别比对照组增加了2倍、8倍和14倍。这些结果表明，蛋白激酶C在神经细胞分化的控制中起关键作用，其特异性抑制可能具有基础和实际重要性。
