Large Animals Clinical Department, Universidade Federal de Santa Maria, Roraima Avenue 1000, Veterinary Hospital, 97105900, Santa Maria, RS, Brazil.
Vet Res Commun. 2010 Aug;34(6):549-55. doi: 10.1007/s11259-010-9426-x. Epub 2010 Jul 5.
The objective of this study was to evaluate oxidative stress in lambs experimentally infected with Haemonchus contortus and supplemented with selenium and vitamin E. Twenty male Corriedale lambs were divided into four experimental groups with five animals each: G1 consisted of animals infected and supplemented with 0.2 mg/kg of live weight (LW) sodium selenite by intramuscular injection (IM); G2 consisted of animals infected with larvae and supplemented with 0.2 mg/kg LW sodium selenite IM and 2000 IU per animal of Vitamin E IM; G3 consisted of animals infected with larvae and supplemented with 2000 IU per animal of Vitamin E IM; and G4 consisted of animals infected with larvae. The animals were infected orally with 500 H. contortus larvae (L3) every 48 hours for 20 days. For biochemical analyses and eggs per grams of feces (EPG) evaluation, blood and feces were both collected at zero (T0), 20 (T1), 40 (T2) and 60 (T3) days. The weight of the animals was also measured at these times. Lower TBARS values were observed in the supplemented animals compared to the control group. The groups supplemented with Selenium exhibited blood GSH-Px activity higher than that of non-supplemented animals. Supplementation with selenium provided greater antioxidant protection against oxidative stress generated from experimental infection of lambs with H. contortus.
本研究旨在评估感染捻转血矛线虫的羔羊的氧化应激，并补充硒和维生素 E。20 只雄性 Corriedale 羔羊被分为四个实验组，每组 5 只：G1 组由肌肉注射（IM）0.2mg/kg 活体重（LW）亚硒酸钠的感染动物组成；G2 组由感染幼虫并 IM 补充 0.2mg/kg LW 亚硒酸钠和 IM 每只动物 2000IU 维生素 E 的动物组成；G3 组由 IM 补充 2000IU 维生素 E 的感染幼虫动物组成；G4 组由感染幼虫的动物组成。动物在 20 天内每隔 48 小时口服感染 500 条捻转血矛线虫（L3）。为了进行生化分析和粪便卵数（EPG）评估，在 0（T0）、20（T1）、40（T2）和 60（T3）天采集血液和粪便。在这些时间点还测量了动物的体重。与对照组相比，补充组的 TBARS 值较低。补充硒的组的血液 GSH-Px 活性高于未补充的动物。补充硒对捻转血矛线虫感染羔羊产生的氧化应激提供了更大的抗氧化保护。
