Hussain M, Lenard J
Department of Physiology and Biophysics, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Rutgers University, Piscataway 08854.
Gene. 1991 May 15;101(1):149-52. doi: 10.1016/0378-1119(91)90238-7.
PDR4 is a gene that confers pleiotropic drug resistance (pdr) to the yeast Saccharomyces cerevisiae when present in high copy number [Leppert et al., Genetics 125 (1990) 13-20]. Transposon insertion mutations had identified the active region of the gene as a 3.7-kb SalI-EcoRI restriction fragment of the 8-kb cloned fragment. We have confirmed this by showing that this fragment is sufficient to confer pdr, and have sequenced its entire 3761 bp. It contains a single complete open reading frame (ORF) extending from nucleotide (nt) position 1631-3580, coding for a protein of 650 amino acids (aa). A 2.7-kb fragment containing this ORF is also sufficient to confer pdr. The aa sequence contains no recognizable homologies or consensus sequences, so it is a novel protein of unknown function. It is apparently soluble, since no transmembrane-type sequences were predicted. A second, partial ORF was also found, on the opposite strand, extending from nt position 774 to past the SalI site, which is apparently unrelated to pdr.
PDR4是一种基因，当以高拷贝数存在时，它赋予酿酒酵母多药耐药性（pdr）[Leppert等人，《遗传学》125（1990）13 - 20]。转座子插入突变已将该基因的活性区域鉴定为8 kb克隆片段中的一个3.7 kb SalI - EcoRI限制片段。我们通过证明该片段足以赋予pdr来证实了这一点，并对其整个3761 bp进行了测序。它包含一个单一的完整开放阅读框（ORF），从核苷酸（nt）位置1631延伸至3580，编码一个650个氨基酸（aa）的蛋白质。包含该ORF的一个2.7 kb片段也足以赋予pdr。该氨基酸序列不包含可识别的同源性或共有序列，因此它是一种功能未知的新型蛋白质。由于未预测到跨膜型序列，它显然是可溶的。在相反链上还发现了第二个部分ORF，从nt位置774延伸至超过SalI位点，这显然与pdr无关。
