对酿酒酵母第十一染色体左臂上一个3.5千碱基对的EcoRI片段进行序列分析，揭示了MBR1基因的位置以及一个与GTP酶激活蛋白相关的序列。
Sequence analysis of a 3.5 Kb EcoRI fragment from the left arm of Saccharomyces cerevisiae chromosome XI reveals the location of the MBR1 gene and a sequence related to a GTPase-activating protein.
作者信息
James C M, Gent M E, Oliver S G
机构信息
Manchester Biotechnology Centre, University of Manchester Institute of Science and Technology (UMIST), U.K.
出版信息
Yeast. 1994 Feb;10(2):257-64. doi: 10.1002/yea.320100212.
We present the DNA sequence analysis of a region covering a 3.5 kb EcoRI fragment from the left arm of chromosome XI from Saccharomyces cerevisiae. This region contains five open reading frames (ORFs) which code for proteins of greater than 100 amino acids. ORF YKL425 codes for the previously sequenced Mbr1 (Valens et al., 1991; Daignan-Fornier et al., 1993) which participates in mitochondrial biogenesis. YKL424 has identity with a GTPase-activating protein of higher eukaryotes. The three remaining ORFs have no identity to known proteins within the databases screened and are not assigned ORF numbers as they are completely contained with ORFs YKL424 and YKL425.
我们展示了对酿酒酵母（Saccharomyces cerevisiae）第十一条染色体左臂上一个3.5 kb EcoRI片段区域的DNA序列分析。该区域包含五个开放阅读框（ORF），它们编码的蛋白质长度超过100个氨基酸。开放阅读框YKL425编码先前已测序的Mbr1（瓦伦斯等人，1991年；戴尼南 - 福尔尼尔等人，1993年），其参与线粒体生物合成。YKL424与高等真核生物的一种GTP酶激活蛋白具有同源性。其余三个开放阅读框在筛选的数据库中与已知蛋白质无同源性，且由于它们完全包含在开放阅读框YKL424和YKL425内，因此未被赋予开放阅读框编号。
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