School of Physical Education and Sport of Ribeirão Preto, University of São Paulo, Av. Bandeirantes, 3900-Monte Alegre, Ribeirão Preto, São Paulo, 14040-900, Brazil.
Eur J Appl Physiol. 2010 Nov;110(4):825-32. doi: 10.1007/s00421-010-1568-6. Epub 2010 Jul 8.
The polymorphisms of endothelial nitric oxide synthase (eNOS) are associated with reduced eNOS activity. Aerobic exercise training (AEX) may influence resting nitric oxide (NO) production, oxidative stress and blood pressure. The purpose of this study was to investigate the effect of AEX on the relationship among blood pressure, eNOS gene polymorphism and oxidative stress in pre-hypertensive older people. 118 pre-hypertensive subjects (59 ± 6 years) had blood samples collected after a 12 h overnight fast for assessing plasma NO metabolites (NOx) assays, thiobarbituric acid reactive substances (T-BARS) and superoxide dismutase activity (ecSOD). eNOS polymorphism (T-786C and G-894T) was done by standard PCR methods. All people were divided according to the genotype results (G1: TT/GG, G2: TT/GT + TT, G3: TC + CC/GG, G4: TC + CC/GT + TT). All parameters were measured before and after 6 months of AEX (70% of VO(2 max)). At baseline, no difference was found in systolic and diastolic blood pressure, ecSOD and T-BARS activity. Plasma NOx levels were significantly different between G1 (19 ± 1 μM) and G4 (14.2 ± 0.6 μM) and between G2 (20.1 ± 1.7 μM) and G4 (14.2 ± 0.6 μM). Therefore, reduced NOx concentration in G4 group occurred only when the polymorphisms were associated, suggesting that these results are more related to genetic factors than NO-scavenging effect. After AEX, the G4 increased NOx values (17.2 ± 1.2 μM) and decreased blood pressure. G1, G3 and G4 decreased T-BARS levels. These results suggest the AEX can modulate the NOx concentration, eNOS activity and the relationship among eNOS gene polymorphism, oxidative stress and blood pressure especially in C (T-786C) and T (G-894T) allele carriers.
内皮型一氧化氮合酶（eNOS）的多态性与 eNOS 活性降低有关。有氧运动训练（AEX）可能影响静息一氧化氮（NO）的产生、氧化应激和血压。本研究的目的是探讨 AEX 对高血压前期老年人血压、eNOS 基因多态性与氧化应激之间关系的影响。118 名高血压前期患者（59 ± 6 岁）在禁食 12 小时后采集血液样本，用于评估血浆 NO 代谢产物（NOx）、硫代巴比妥酸反应物质（T-BARS）和超氧化物歧化酶活性（ecSOD）。eNOS 多态性（T-786C 和 G-894T）通过标准 PCR 方法进行。根据基因型结果将所有人分为 4 组（G1：TT/GG，G2：TT/GT + TT，G3：TC + CC/GG，G4：TC + CC/GT + TT）。所有参数在 AEX（70% VO（2 max））前后 6 个月进行测量。在基线时，收缩压和舒张压、ecSOD 和 T-BARS 活性没有差异。G1（19 ± 1 μM）和 G4（14.2 ± 0.6 μM）之间以及 G2（20.1 ± 1.7 μM）和 G4（14.2 ± 0.6 μM）之间的血浆 NOx 水平有显著差异。因此，只有当多态性相关时，G4 组的 NOx 浓度才会降低，这表明这些结果与遗传因素比 NO 清除作用更相关。AEX 后，G4 组的 NOx 值增加（17.2 ± 1.2 μM），血压降低。G1、G3 和 G4 组 T-BARS 水平降低。这些结果表明，AEX 可以调节 NOx 浓度、eNOS 活性以及 eNOS 基因多态性、氧化应激与血压之间的关系，特别是在 C（T-786C）和 T（G-894T）等位基因携带者中。
