Department of Biology and Environmental Science, School of Life Sciences, University of Sussex, Falmer, Brighton BN1 9QJ, UK.
Environ Pollut. 2010 Sep;158(9):2977-84. doi: 10.1016/j.envpol.2010.05.025. Epub 2010 Jul 7.
Mytilus edulis were exposed to 17beta-estradiol (E2) and the synthetic estrogens ethinyl estradiol (EE2) and estradiol benzoate (EB) for 10 days. Two exposures were performed to determine their effect on vitellogenin (VTG) and estrogen receptor 2 (ER2) mRNA expression at different stages of the reproductive cycle. Significant natural variation was not observed in VTG mRNA expression, though ER2 mRNA expression displayed significantly lower values during January, February and July compared with other times of the year. A significant increase in VTG and ER2 mRNA expression was observed in mussels exposed to estrogens at the early stage of gametogenesis. In contrast, mature mussels displayed no statistically significant change in the VTG or ER2 mRNA expression. The data presented suggests that the reproductive physiology of molluscs, in terms of VTG and ER2 mRNA expression, may be susceptible to damage by environmental estrogens at certain points in their gametogenesis process.
贻贝暴露于 17β-雌二醇（E2）和合成雌激素乙炔雌二醇（EE2）和苯甲酸雌二醇（EB）中 10 天。进行了两次暴露，以确定它们对卵黄蛋白原（VTG）和雌激素受体 2（ER2）mRNA 表达在生殖周期不同阶段的影响。尽管 ER2 mRNA 表达在 1 月、2 月和 7 月与一年中的其他时间相比显示出显著较低的值，但 VTG mRNA 表达未观察到明显的自然变异。在配子发生的早期阶段暴露于雌激素的贻贝中，观察到 VTG 和 ER2 mRNA 表达的显著增加。相比之下，成熟贻贝的 VTG 或 ER2 mRNA 表达没有统计学上的显著变化。所提出的数据表明，就 VTG 和 ER2 mRNA 表达而言，贝类的生殖生理学可能容易受到其配子发生过程中某些点的环境雌激素的损害。
