Zhang Dandan, Fang Jianguo, Chen Juanjuan, Wan Jin, Zhang Yawei, Yin Zongzhi, Wang Nan, Chen Suhua
Department of Obstetrics and Gynecology, Tongli Hospital Affiliated with Tongfi Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhongguo Zhong Yao Za Zhi. 2010 Apr;35(8):1055-9.
To study the in vitro anti-human cytomegalovirus effect and the cytotoxicity of Forsythia suspensa and its main active ingredient quercetin.
The 0% toxic dose (TD0), minimum effective concentration (MEC) and therapeutic index (TI) of anti-human cytomegalovirus activity by F. suspensa and quercetin were detected with the cytopathic assay and MTT method. Ganciclovir was used as the control drug for comparison.
The TD0 of ganciclovir, F. suspensa and quercetin were 10, 30, 30 mg L(-1), the MEC were 10, 30, 0.3 mg x L(-1), TI were 1, 1 and 100, respectively.
The anti-human cytomegalovirus effect of quercetin is much higher than ganciclovir and F. suspensa, and the cytotoxicity is equivalent to F. suspensa but lower than ganciclovir. Therefore, quercetin has the potential advantages of anti-human cytomegalovirus effect.
研究连翘及其主要活性成分槲皮素的体外抗人巨细胞病毒作用及细胞毒性。
采用细胞病变法和MTT法检测连翘和槲皮素抗人巨细胞病毒活性的零毒性剂量（TD0）、最小有效浓度（MEC）和治疗指数（TI）。更昔洛韦作为对照药物进行比较。
更昔洛韦、连翘和槲皮素的TD0分别为10、30、30 mg·L⁻¹，MEC分别为10、30、0.3 mg·L⁻¹，TI分别为1、1、100。
槲皮素的抗人巨细胞病毒作用远高于更昔洛韦和连翘，细胞毒性与连翘相当但低于更昔洛韦。因此，槲皮素具有抗人巨细胞病毒作用的潜在优势。
