Chen Yang, Li Xin, Zhou Jing-Yu, Yang Qing-Wei, Li Hongyuan
Public Health College of Harbin Medical University, Harbin 150081, China.
Wei Sheng Yan Jiu. 2009 Nov;38(6):733-5.
To study the extraction of an active component LC-4 from Forsythia suspensa (Thunb.) Vahl and anti-respiratory syncytial virus (RSV) effect in vitro.
The extration from boiling water of Forsythia suspensa were extracted by hot water extraction and were precipitated by ethanol and were elicited by macroporous adsorptive resins column chromatography. Then the antiviral active component LC-4 was separated from the production by eluting. The cytotoxicities and antiviral effect of LC-4 were tested by the Neutral Red assay and the cytopathic effect (CPE) inhibition assay. Ribavirin was used as the positive drug in the study. The antiviral effect of prevention and treatment of maximum dose of non-cytotoxicity of LC-4 was tested in Hela cell line.
LC-4 showed that the concentration were 25.4 microg/ml with the medium cytotoxicity and were 2.11 microg/ml with the medium effective concentration (EC50). The corresponding treatment index of LC-4 was 12.04. It also exhibited obviously inhibition effect on RSV replications when LC-4 was added at 2 h, 4 h, 6 h and 8 h after infection (P < 0.05). The CPE was significant difference in comparison with control group, when the concentration of LC-4 between 31.25 microg/ml and 3.91 microg/ml was added at 2 h before infection (P < 0.05).
LC-4 could significantly prevent and inhibit RSV replications in vitro.
研究从连翘中提取活性成分LC - 4及其体外抗呼吸道合胞病毒（RSV）的作用。
采用热水提取连翘水煮液，乙醇沉淀，大孔吸附树脂柱色谱法分离得到抗病毒活性成分LC - 4。通过中性红试验和细胞病变效应（CPE）抑制试验检测LC - 4的细胞毒性和抗病毒作用。本研究以利巴韦林作为阳性对照药。在Hela细胞系中检测LC - 4最大非细胞毒性剂量的预防和治疗抗病毒效果。
LC - 4的细胞毒性中浓度为25.4μg/ml，有效浓度（EC50）为2.11μg/ml。LC - 4相应的治疗指数为12.04。感染后2 h、4 h、6 h和8 h加入LC - 4时，对RSV复制也有明显抑制作用（P < 0.05）。感染前2 h加入浓度为31.25μg/ml至3.91μg/ml的LC - 4时，CPE与对照组相比有显著差异（P < 0.05）。
LC - 4在体外能显著预防和抑制RSV复制。
