Division of Medicine, Indian Veterinary Research Institute, Izatnagar, Bareilly, UP, India.
Parasitology. 2010 Nov;137(13):1921-4. doi: 10.1017/S0031182010000879. Epub 2010 Jul 12.
The aim of this study was to evaluate the CD4+/CD8+ ratio in peripheral blood of dogs with localized and generalized demodicosis. Sixteen dogs were examined, 8 with localized and 8 with generalized demodicosis, while 8 healthy dogs were used as controls. Peripheral blood was obtained and CD4+ and CD8+ T cells were determined by flow cytometry. Significantly higher numbers of CD8+ T cells and lower numbers of CD4+ T cells were found in dogs with generalized demodicosis compared to dogs with localized demodicosis and healthy controls. Significantly higher numbers of CD8+ T cells and lower numbers of CD4+ T cells were also found in dogs with localized demodicosis compared to healthy controls. The CD4+/CD8+ ratio was also found to be significantly lower in dogs with generalized demodicosis in comparison with dogs with localized demodicosis and healthy controls. It is concluded that significant alteration in the CD4+/CD8+ ratio may be implicated in the pathogenesis of generalized canine demodicosis.
本研究旨在评估局限性和全身性蠕形螨病犬外周血 CD4+/CD8+ 比值。共检查了 16 只狗，8 只为局限性蠕形螨病，8 只为全身性蠕形螨病，同时使用 8 只健康狗作为对照。采集外周血，通过流式细胞术测定 CD4+和 CD8+T 细胞。与局限性蠕形螨病和健康对照组相比，全身性蠕形螨病犬的 CD8+T 细胞数量显著增加，而 CD4+T 细胞数量显著减少。与健康对照组相比，局限性蠕形螨病犬的 CD8+T 细胞数量也显著增加，而 CD4+T 细胞数量显著减少。与局限性蠕形螨病和健康对照组相比，全身性蠕形螨病犬的 CD4+/CD8+ 比值也显著降低。因此，全身性犬蠕形螨病的发病机制可能与 CD4+/CD8+ 比值的显著改变有关。
