Oliveira Camila D, Larsson Carlos E, de Camargo Maristela M
Department of Clinical Sciences, School of Veterinary Medicine, University of São Paulo, Av Prof. Orlando M. Paiva 87, Sao Paulo, SP, 05508-270, Brazil.
Vet Dermatol. 2015 Feb;26(1):18-22, e5-6. doi: 10.1111/vde.12183. Epub 2014 Oct 30.
The pathogenesis of canine generalized demodicosis is poorly understood but is thought to involve dysfunction of the immune system. Previous studies showed diminished CD4+ T lymphocyte counts in affected dogs, but none has evaluated this subpopulation through resolution of the disease.
HYPOTHESIS/OBJECTIVES: In this longitudinal study, we tested whether quantification of CD4+ cells, CD8+ cells and the ratio of CD4+ to CD8+ cells are good indicators of immunological status and could be used as biomarkers of treatment efficacy and prognosis.
Sixteen dogs of several breeds with diagnoses of generalized demodicosis, plus 30 age/breedmatched healthy dogs.
Total lymphocytes, CD4+, CD8+ and CD4+:CD8+ ratio were quantified at four time points: at diagnosis, 30 days after diagnosis (during treatment), at first negative parasitological examination and at clinical cure.
Absolute numbers of CD4+ cells were significantly lower in affected dogs at the time of diagnosis. Absolute numbers of CD4+ and CD8+ cells were significantly augmented in affected animals compared with control dogs after treatment was established, and this persisted until the first negative parasitological examination, at which time the CD4+ counts equalled those of the control group.
Our findings suggest that longitudinal quantification of CD4+ and CD8+ T lymphocytes is a useful indicator of the efficacy of demodicosis treatment.
犬全身性蠕形螨病的发病机制尚不清楚，但认为与免疫系统功能障碍有关。先前的研究表明，患病犬的CD4+ T淋巴细胞计数减少，但尚无研究通过疾病的缓解过程评估这一亚群。
假设/目的：在这项纵向研究中，我们测试了CD4+细胞、CD8+细胞的定量以及CD4+与CD8+细胞的比例是否是免疫状态的良好指标，能否用作治疗效果和预后的生物标志物。
16只诊断为全身性蠕形螨病的不同品种犬，外加30只年龄/品种匹配的健康犬。
在四个时间点对总淋巴细胞、CD4+、CD8+以及CD4+：CD8+比例进行定量：诊断时、诊断后30天（治疗期间）、首次寄生虫学检查阴性时以及临床治愈时。
诊断时，患病犬的CD4+细胞绝对数量显著降低。与对照犬相比，患病动物在治疗开始后CD4+和CD8+细胞的绝对数量显著增加，且这种情况持续到首次寄生虫学检查阴性时，此时CD4+细胞计数与对照组相等。
我们的研究结果表明CD4+和CD8+ T淋巴细胞的纵向定量是蠕形螨病治疗效果的有用指标。
