Caswell J L, Yager J A, Parker W M, Moore P F
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Canada.
Vet Pathol. 1997 Jul;34(4):279-87. doi: 10.1177/030098589703400403.
Mural folliculitis is a consistent histologic lesion of canine demodicosis. The objective of this study was to describe the immunophenotype and to evaluate temporal changes in histologic lesions of demodicosis during the course of therapy. Five dogs with demodicosis were examined and biopsied biweekly for up to 14 weeks; three dogs were evaluated once only. Lymphocyte subsets infiltrating the lesions were quantified using immunohistochemistry to detect CD3, CD21, CD4, and CD8 antigens. Lymphocyte subsets in blood were analyzed from four dogs using flow cytometry. Mural folliculitis was always present during clinically active disease. In contrast, following resolution of clinical lesions, perifolliculitis and/or perifollicular granulomas were present but mural folliculitis was absent. Most lymphocytes infiltrating the follicular epithelium in lesions of mural folliculitis were CD3+ and CD8+; the ratio of CD4+ :CD8+ cells in this epithelium was 0.032. In contrast, the perifollicular dermis contained approximately equal numbers of CD4+ cells and CD8+ cells, with slightly fewer CD21+B cells. In peripheral blood, the ratio of CD4+:CD8+ lymphocytes was reduced and the percentage of CD8+ cells was increased in three of four dogs. These results indicate that mural folliculitis is a consistent lesion of clinically active canine demodicosis and is characterized by infiltration of the follicular epithelium by CD3+ CD8+ T lymphocytes. These lymphocytes are cytotoxic T cells, which may mediate the injury to the follicular epithelium in demodicosis. Alternatively, CD8+ T cells may play a role in resistance to Demodex canis infection or may represent a deleterious immune response in dogs that develop demodicosis.
毛囊壁毛囊炎是犬蠕形螨病持续存在的组织学病变。本研究的目的是描述免疫表型，并评估治疗过程中蠕形螨病组织学病变的时间变化。对5只患有蠕形螨病的犬进行检查，并每两周进行一次活检，持续14周；对3只犬只进行了一次评估。使用免疫组织化学检测CD3、CD21、CD4和CD8抗原，对浸润病变的淋巴细胞亚群进行定量。使用流式细胞术分析了4只犬血液中的淋巴细胞亚群。在临床活动期疾病期间，毛囊壁毛囊炎始终存在。相比之下，临床病变消退后，存在毛囊周炎和/或毛囊周肉芽肿，但不存在毛囊壁毛囊炎。在毛囊壁毛囊炎病变中浸润毛囊上皮的大多数淋巴细胞为CD3+和CD8+；该上皮中CD4+：CD8+细胞的比例为0.032。相比之下，毛囊周真皮中CD4+细胞和CD8+细胞数量大致相等，CD21+B细胞略少。在4只犬中的3只犬外周血中，CD4+：CD8+淋巴细胞的比例降低，CD8+细胞的百分比增加。这些结果表明，毛囊壁毛囊炎是临床活动期犬蠕形螨病的持续病变，其特征是CD3+CD8+T淋巴细胞浸润毛囊上皮。这些淋巴细胞是细胞毒性T细胞，可能介导蠕形螨病中毛囊上皮的损伤。或者，CD8+T细胞可能在抵抗犬蠕形螨感染中起作用，或者可能代表发生蠕形螨病的犬的有害免疫反应。
