Kalia Jeet, Raines Ronald T
Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, Wisconsin 53706-1544, USA.
Curr Org Chem. 2010 Jan;14(2):138-147. doi: 10.2174/138527210790069839.
Bioconjugation is a burgeoning field of research. Novel methods for the mild and site-specific derivatization of proteins, DNA, RNA, and carbohydrates have been developed for applications such as ligand discovery, disease diagnosis, and high-throughput screening. These powerful methods owe their existence to the discovery of chemoselective reactions that enable bioconjugation under physiological conditions-a tremendous achievement of modern organic chemistry. Here, we review recent advances in bioconjugation chemistry. Additionally, we discuss the stability of bioconjugation linkages-an important but often overlooked aspect of the field. We anticipate that this information will help investigators choose optimal linkages for their applications. Moreover, we hope that the noted limitations of existing bioconjugation methods will provide inspiration to modern organic chemists.
生物共轭是一个新兴的研究领域。针对蛋白质、DNA、RNA和碳水化合物的温和且位点特异性衍生化，已开发出了新方法，用于配体发现、疾病诊断和高通量筛选等应用。这些强大的方法得益于化学选择性反应的发现，该反应能够在生理条件下实现生物共轭——这是现代有机化学的一项巨大成就。在此，我们综述了生物共轭化学的最新进展。此外，我们还讨论了生物共轭连接键的稳定性——这是该领域一个重要但常被忽视的方面。我们预计这些信息将有助于研究人员为其应用选择最佳的连接键。此外，我们希望所指出的现有生物共轭方法的局限性能为现代有机化学家提供灵感。
