Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, 208016 Kanpur, India.
J Mater Sci Mater Med. 2010 Nov;21(11):2937-45. doi: 10.1007/s10856-010-4124-3. Epub 2010 Jul 13.
The thermoresponsive poly(N-vinylcaprolactam) (PVCl) based cryogel network were synthesized and characterized with respect to physical and biological properties. The PVCl cryogel crosslinked with polyethylene glycol-diacrylate (PEGda) was synthesized in 1% dimethyl sulfoxide containing aqueous medium at -12°C for 12-14 h. The cryogel synthesized in this manner were highly spongy in nature and can absorb water in its porous network. These polymeric cryogel networks have good physical morphology as confirmed by scanning electron microscopy. The estimated porosity of these cryogels was 90% as demonstrated by various methods based on absorption of water and cyclohexane. The median pore diameter and surface area was 30 μm and 2.0253 m(2)/g, respectively as confirmed by analysis on mercury porosimeter. These materials can interact with biological system without any cytotoxic effects. Change in temperature influenced the adsorption of fetal bovine serum (FBS) on PVCl scaffold which showed maximum protein adsorption at 37°C, as compared to that at 25°C. Furthermore, the fibroblast cell adhesion studies showed the potential of these PVCl based cryogels as tissue engineering scaffolds.
采用聚乙二醇二丙烯酸酯（PEGda）对 N-乙烯基己内酰胺（PVCl）基温敏性水凝胶网络进行交联，在 -12°C 下于含 1%二甲基亚砜的水性介质中反应 12-14 h 来合成水凝胶。这种方式合成的水凝胶具有高度海绵状的特性，可以在其多孔网络中吸收水分。扫描电子显微镜证实了这些聚合物水凝胶网络具有良好的物理形态。通过基于水和环己烷吸收的各种方法，这些水凝胶的估计孔隙率为 90%。通过压汞仪分析，证实了这些水凝胶的中值孔径和比表面积分别为 30 μm 和 2.0253 m(2)/g。这些材料与生物系统相互作用而没有任何细胞毒性。温度的变化会影响胎牛血清（FBS）在 PVCl 支架上的吸附，与 25°C 相比，37°C 时蛋白质的吸附量最大。此外，成纤维细胞黏附研究表明，这些基于 PVCl 的水凝胶具有作为组织工程支架的潜力。
